法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (6): 446-451.DOI: 10.3969/j.issn.1004-5619.2014.06.011

• 论著 • 上一篇    下一篇

Goldeneye®DNA身份鉴定系统26Y试剂盒的法医学验证

阙庭志，林  源，赵珍敏，柳  燕，张素华   

  1. （司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2014-12-25 出版日期:2014-12-28
  • 通讯作者: 张素华，女，助理研究员，主要从事法医遗传学研究；E-mail：zsh-daisy@163.com
  • 作者简介:阙庭志（1963—），男，上海人，副主任法医师，主要从事法医物证学研究；E-mail：quetz@ssfjd.cn
  • 基金资助:

    国家自然科学基金资助项目（81302620）；“十二五”国家科技支撑计划项目（2012BAK16B01）

Forensic Validation of Goldeneye®DNA ID 26Y System

QUE TING-ZHI, LIN YUAN, ZHAO ZHEN-MIN, LIU YAN, ZHANG SU-HUA   

  1.   （Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2014-12-25 Published:2014-12-28

摘要： 目的 对Goldeneye®DNA身份鉴定系统26Y试剂盒的法医学参数进行验证和分析。 方法 根据DNA分析方法科学工作组（Scientific Working Group on DNA Analysis Methods，SWGDAM）对试剂盒的法医学验证要求，从PCR扩增体系的测试、重复性、准确性、灵敏度等多个角度对该试剂盒进行检测评估。应用该试剂盒对华东地区517名汉族健康无关个体进行Y-STR基因座分型检测，检测单倍型分布状况及频率信息，并评估该试剂盒的法医学参数。 结果 该试剂盒对6.25 ?滋L扩增体系、DNA量低至125 pg时仍然可以得到准确的分型结果。特异性检测发现该试剂盒对常见的动物DNA和微生物DNA无有效的扩增结果。男性混合样本（1∶19和19∶1）中，较少样本的等位基因检出率可以达到70%以上；在男女混合样本中，女性DNA背景的存在不影响试剂盒的灵敏度。 结论 Goldeneye®DNA身份鉴定系统26Y试剂盒灵敏度高、特异性好，且可以应用于混合物的检测。试剂盒所包含的26个Y-STR基因座多态性良好，可满足法医学实际应用。

关键词:  , 法医遗传学, Y-STR, Goldeneye?誖DNA身份鉴定系统26Y试剂盒

Abstract: Objective To perform the validation and analysis of forensic parameters of Goldeneye®DNA ID 26Y system. Methods Based on the validation rules of Scientific Working Group on DNA Analysis Methods （SWGDAM）, the kit was assessed from several parts, as test of PCR system, reproducibility, accuracy, and sensitivity, etc. And Y-STR loci of 517 unrelated healthy individuals from Eastern China were genotypes by this kit. The distribution and frequency of haplotype were calculated and forensic parameters of the kit were assessed. Results The complete profiles can be obtained even when the PCR reaction volume with 6.25 μL. And correct profile was obtained with DNA down to 125 pg. No reproducible peaks were detected with the DNA of common animals and microorganism with the kit. For the male-male mixture testing, average 70% of the minor alleles were obtained when the ratios of 1∶19 and 19∶1. For the male-female mixture testing, results showed that the sensitivity of the kit was no compromised with the addition of female samples. Conclusion The validation studies demonstrated that Goldeneye®DNA ID 26Y system has good sensitivity and specificity, and suitable for mixture testing. The polymorphism of 26 Y-STR loci included in this kit are good for forensic application.

Key words: forensic genetics, Y-STR, Goldeneye?誖DNA ID 26Y system

中图分类号: