法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (2): 136-140.DOI: 10.3969/j.issn.1004-5619.2017.02.006

• 论著 • 上一篇    下一篇

IBS评分法鉴定全同胞关系及其临界值查询表的构建

李  燃1，李成涛2，赵书民3，李海霞1，李  莉2，乌日嘎1，张楚楚1，孙宏钰1   

  1. （1. 中山大学中山医学院法医学系，广东 广州 510089； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台，上海 200063； 3. 上海五色石医学研究股份有限公司，上海 201200）
  • 发布日期:2017-04-25 出版日期:2017-04-28
  • 通讯作者: 孙宏钰，女，博士，教授，主要从事法医物证学研究；E-mail：sunhongyu2002@163.com
  • 作者简介:李燃（1991—），男，硕士研究生，主要从事法医物证学研究；E-mail：liran654@qq.com
  • 基金资助:

    国家自然科学基金面上项目（81273347，81671873）；上海市法医学重点实验室资助项目（17DZ2273200）；上海市司法鉴定专业技术服务平台资助项目（16DZ2290900）

Full Sibling Identification by IBS Scoring Method and Establishment of the Query Table of Its Critical Value

LI RAN1, LI CHENG-TAO2, ZHAO SHU-MIN3, LI HAI-XIA1, LI LI2, WU RI-GA1, ZHANG CHU-CHU1, SUN HONG-YU1   

  1. （1. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China; 3. Shanghai Chromysky Medical Research Co., Ltd, Shanghai 201200, China）
  • Online:2017-04-25 Published:2017-04-28

被引次数

12

摘要： 目的 建立包含不同个数STR基因座检测体系在不同错判标准下的IBS临界值和检测效能查询表。 方法 收集267对全同胞和360对无关个体血样，采用GoldeneyeTM 20A体系进行19个常染色体STR基因座的分型，按照《生物学全同胞关系鉴定实施规范》采用IBS评分法判定全同胞关系。通过理论推算，计算包含不同个数STR基因座检测体系在不同错判标准下的IBS临界值和检测效能。 结果 按照规范的IBS评分标准对全同胞和无关个体的鉴别效能为0.764 0，错判率为0，理论推算和样本观察值相符。包含不同个数STR基因座检测体系的全同胞鉴定IBS评分临界值查询表构建成功。 结论 该规范的IBS评分法检测效力较高，错判率极低，判定结果相对保守。包含不同个数STR基因座检测体系的全同胞鉴定IBS评分临界值查询表为全同胞鉴定的结果评判提供了重要的参考数据，具有很好的应用价值。

关键词: 法医遗传学, 同胞关系, 短串联重复序列, 状态一致性

Abstract: Objective To establish a query table of IBS critical value and identification power for the detection systems with different numbers of STR loci under different false judgment standards. Methods Samples of 267 pairs of full siblings and 360 pairs of unrelated individuals were collected and 19 autosomal STR loci were genotyped by GoldeneyeTM 20A system. The full siblings were determined using IBS scoring method according to the ‘Regulation for biological full sibling testing’. The critical values and identification power for the detection systems with different numbers of STR loci under different false judgment standards were calculated by theoretical methods. Results According to the formal IBS scoring criteria, the identification power of full siblings and unrelated individuals was 0.764 0 and the rate of false judgment was 0. The results of theoretical calculation were consistent with that of sample observation. The query table of IBS critical value for identification of full sibling detection systems with different numbers of STR loci was successfully established. Conclusion The IBS scoring method defined by the regulation has high detection efficiency and low false judgment rate, which provides a relatively conservative result. The query table of IBS critical value for identification of full sibling detection systems with different numbers of STR loci provides an important reference data for the result judgment of full sibling testing and owns a considerable practical value.

Key words: forensic genetics, sibling relations, short tandem repeat, identity by state （IBS）

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