法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (5): 476-481.DOI: 10.3969/j.issn.1004-5619.2017.05.004

所属专题： 心脏性猝死

• 专题 • 上一篇    下一篇

proBNP和NT-proBNP在冠心病猝死者体内的表达

曾  强1，孙润峰1，李  泽1，2，翟利琴1，2，刘明哲1，郭相杰1，高彩荣1   

  1. 1. 山西医科大学法医学院法医病理教研室，山西 太原 030001； 2. 山西省人民医院，山西 太原 030012
  • 发布日期:2017-10-25 出版日期:2017-10-28
  • 通讯作者: 高彩荣，女，博士，博士研究生导师，主要从事猝死病理学教学研究和检案；E-mail：gaocairong5175@163.com
  • 作者简介:曾强（1992—），男，硕士研究生，主要从事猝死病理学研究；E-mail：zeng_smile@126.com
  • 基金资助:

    ：山西省科技攻关资助项目（20140313015-7）；山西省回国留学人员科研资助项目（2015-050）

Expression of proBNP and NT-proBNP in Sudden Death of Coronary Heart Disease

ZENG QIANG1, SUN RUI-FENG1, LI ZE1,2, ZHAI LI-QIN1,2, LIU MING-ZHE1, GUO XIANG-JIE1, GAO CAI-RONG1   

  1. 1. Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Shanxi Provincial People’s Hospital, Taiyuan 030012, China
  • Online:2017-10-25 Published:2017-10-28

被引次数

3

摘要： 目的 研究冠状动脉粥样硬化性心脏病（以下简称冠心病）猝死者体内脑钠肽前体（pro-brain natriuretic peptide，proBNP）、N端脑钠肽前体（N-terminal pro-brain natriuretic peptide，NT-proBNP）的表达变化，探讨其在冠心病猝死法医学诊断中的应用价值。 方法 取正常对照组、冠心病猝死组及单独冠状动脉狭窄组（每组各20例）心肌及心血样本。运用免疫组织化学染色、Western印迹法检测心肌样本中proBNP的表达，采用逆转录PCR（reverse transcription PCR，RT-PCR）技术检测BNP mRNA的表达，采用ELISA法检测血浆中NT-proBNP的含量。 结果 冠心病猝死组、单独冠状动脉狭窄组心肌组织中proBNP免疫组织化学染色均为阳性表达，正常对照组无阳性染色。冠心病猝死组、单独冠状动脉狭窄组心肌组织内proBNP蛋白、BNP mRNA相对表达量、血浆内NT-proBNP的含量较正常对照组升高（P<0.05）。冠心病猝死组血浆内NT-proBNP含量高于单独冠状动脉狭窄组（P<0.05）。 结论 心肌缺血时心肌细胞内的proBNP表达升高，测定血浆中NT-proBNP有助于判断冠心病的严重程度及鉴别冠心病猝死。

关键词: 法医病理学, 猝死, 冠心病, 脑钠肽前体, N端脑钠肽前体

Abstract: Objective To study the expression change of pro-brain natriuretic peptide （proBNP） and N-terminal pro-brain natriuretic peptide （NT-proBNP） in sudden death of coronary atherosclerotic heart disease, and to explore its application in forensic diagnosis. Methods Myocardial and blood samples were collected from normal control group, sudden death of coronary atherosclerotic heart disease group and single coronary stenosis group （20 cases in each group）. The expression of proBNP in myocardial samples were detected by immunohistochemical staining and Western blotting, and that of BNP mRNA were detected by reverse transcription PCR （RT-PCR）. The content of NT-proBNP in plasma were detected by ELISA. Results Immunohistochemical staining showed positive expression of proBNP in both sudden death of coronary atherosclerotic heart disease group and single coronary stenosis group. There was no positive expression in normal control group. For sudden death of coronary atherosclerotic heart disease group and single coronary stenosis group, the relative expression of proBNP protein and BNP mRNA in myocardial tissue and the NT-proBNP content in plasma were higher than that of normal control group （P<0.05）. The NT-proBNP content in plasma of sudden death of coronary atherosclerotic heart disease group was higher than that of single coronary stenosis group （P<0.05）. Conclusion In myocardial ischemia condition, the higher expression of proBNP in cardiac muscle cell shows that the detection of NT-proBNP in plasma can be useful to differentially diagnose the degree of coronary atherosclerotic heart disease and determine whether the sudden death due to coronary atherosclerotic heart disease.

Key words: forensic pathology, death, sudden, coronary disease, pro-brain natriuretic peptide, N-terminal pro-brain natriuretic peptide

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