法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 39-42.

• 论文 • 上一篇    下一篇

STR滑脱模型的构建及其影响因素

邓建强;李英碧;吴谨;侯一平;   

  1. 司法部司法鉴定科学技术研究所,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院 上海200063四川大学华西基础医学与法医学院,四川成都610041,四川成都610041,四川成都610041,四川成都610041
  • 发布日期:2006-02-25 出版日期:2006-02-28

Constructing of STR Slippage Model by Optimizing Some Factors

DENG JIAN-QIANG1, LI YING-BI2, WU JIN2,HOU YI-PING2 (1.INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063,CHINA ; 2. INSTITUTE OF FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041,CHINA)   

  • Online:2006-02-25 Published:2006-02-28

被引次数

6

摘要： 目的探索建立体外STR滑脱模型,研究STR滑脱产生的影响因素,探讨STR的发生机制。方法首先通过全基因组扩增技术—简并寡核苷酸引物PCR(Degenerateoligonucleotide-primedPCR,DOP)对模板DNA样本进行扩增放大,然后以其产物作为后续STR分析的模板,对以上实验过程中的一系列实验条件进行控制,观察滑脱现象的产生情况。结果初步建立了STR的滑脱模型,观察到了STR滑脱现象的发生。结论STR的发生是一系列综合因素作用的结果,DNA模板用量、MgCl2的浓度、DNA聚合酶的性质、样本以及STR的基序组成等因素均可能参与了这一过程。

关键词: 短串联重复, 滑脱, 模型, 简并寡核苷酸引物

Abstract: Objective To construct STR slippage model and study factors involved in this procedure. Methods DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR ,then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors. Results STR slippage model was constructed. Conclusion Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.

Key words: short tandem repeat(STR), slippage, model, degenerate oligonucleotide- primed PCR