法医学杂志

法医学杂志 ›› 2006, Vol. 22 ›› Issue (1): 45-47.

• 论文 • 上一篇    下一篇

线粒体DNA编码区的多态性

刘雅诚;郝金萍;严江伟;唐晖;王静;任贺;任嘉诚;   

  1. 北京市公安局法医检验鉴定中心,公安部物证鉴定中心,北京市公安局法医检验鉴定中心,北京市公安局法医检验鉴定中心,北京市公安局法医检验鉴定中心,北京人民警察学院,北京市公安局法医检验鉴定中心 北京,100085,北京100038,北京,100085,北京,100085,北京,100085,北京102202山西医科大学法医学院,山西太原030001,北京,100085
  • 发布日期:2006-02-25 出版日期:2006-02-28

Polymorphisms Analysis of Mitochondrial DNA in Coding Area

LIU YA-CHENG1, HAO JIN-PING2, YAN JIAN-GWEI1, TANG HUI1, WANG JING1, REN HE2,3, REN JIA-CHENG1 (1.FORENSIC MEDICAL EXAMINATION CENTER OF BEIJING PUBLIC SECURITY BUREAU,BEIJING 100085,CHINA; 2.SCHOOL OF FORENSIC MEDICINE,SHANXI MEDICAL UNIVERSITY,TAIYUAN 0   

  • Online:2006-02-25 Published:2006-02-28

被引次数

11

摘要： 目的研究线粒体DNA(mtDNA)编码区单核苷酸多态性,建立mtDNA编码区多态性在法庭科学中应用的理论基础。方法针对mtDNA编码区nt8162-8483以及nt13070-13299两段序列设计引物,应用直接测序技术研究其多态性。结果两对引物扩增片段长分别为322bp和230bp,共检测到21种变异,24种单倍型,基因多样性为0.7511,两个无关个体的偶合概率为0.2564。结论线粒体DNA编码区多态性位点作为线粒体DNA控制区多态性位点的补充,联合应用可以提高线粒体DNA在法医学应用中的个体识别能力。

关键词: 线粒体DNA编码区, 单核苷酸多态性, 测序

Abstract: Objective To analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis for applying in forensic science. Methods The primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson′s sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and nt13070-13299. Results The lengths of the amplicons were 322 bp and 230 bp respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 0.751 1, and the probability of two randomly selected individuals having identical mtDNA types was 0.256 4. Conclusion The polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discrimination power.

Key words: mitochondrial DNA, coding area, single nucleotide polymorphisms, sequencing