法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 401-404.

• 论文 • 上一篇    下一篇

小鼠皮肤切创愈合过程中c-jun的表达

杨玫;官大威;熊昌艳;程子惠;于天水;   

  1. 中国医科大学法医学院;江苏大学法医学教研室;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Expression of c-jun During the Incised Wound Healing in Mice Skin

YANG MEI1, GUAN DA-WEI1, XIONG CHANG-YAN1,2, CHENG ZI-HUI1, YU TIAN-SHUI1 (1. SCHOOL OF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY, SHENYANG 110001, CHINA; 2. DEPARTMENT OF FORENSIC MEDICINE, JIANGSU UNIVERSITY, ZHENJIANG 212001, CHINA)   

  • Online:2009-12-25 Published:2009-12-28

被引次数

7

摘要： 目的探讨小鼠皮肤切创愈合过程中损伤区c-jun的表达及不同损伤时间c-jun的变化规律。方法应用免疫组织化学染色和Western印迹技术检测小鼠皮肤切创后各个时间段c-jun的表达变化情况。结果正常组织中有少量c-jun表达。伤后3～12h损伤区c-jun主要表达在中性粒细胞中,1～5dc-jun阳性细胞主要以单个核细胞和成纤维细胞为主,7～14dc-jun主要在成纤维细胞中表达。阳性细胞率3～12h逐渐增高,12h达高峰,1～5d有所下降,7d阳性细胞率达第二峰值,10～14d逐渐降低。Western印迹法检测显示各个时间段均有c-jun阳性条带,其中12h和7d为c-jun含量的2个高峰。结论小鼠皮肤切创愈合过程中c-jun在损伤区对诱导中性粒细胞、单个核细胞及成纤维细胞的凋亡可能起着重要作用,同时c-jun表达的规律性变化可用于损伤时间的推断。

关键词: 法医病理学, 创伤和损伤, 伤口愈合, 基因, jun, 损伤时间, 小鼠

Abstract: Objective To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin. Methods The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot. Results There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12 h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injury. The c-jun positive cells were mostly fibroblasts between 7 d and 14 d after injury. The ratio of the c-jun positive cells increased in the wound specimens from 3 h to 12 h, peaked at 12 h, declined partially from 1 d to 5 d, and reached the peak secondly at 7 d, then decreased from 10 d to 14 d. The expression of c-jun was observed throughout the wound healing stages by Western blot with two peaks occurring at 12 h and 7 d after injury. Conclusion The c-jun may play a potential role in inducing apoptosis of neutrophils, MNCs and fibroblasts during skin wound healing, and it may be used as the marker for wound age determination.

Key words: forensic pathology, wounds and injuries, wound healing, genes, jun, wound age, mice