法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 437-439.

• 论文 • 上一篇    下一篇

血液和尿液中多种头孢菌素的HPLC分析

李牧;廖林川;颜有仪;杨林;刘晓敏;代晶;马晓娜;盛欣;黄红;粟贵;   

  1. 四川大学华西基础医学与法医学院;成都医学院药学院;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Analysis of Multiple Cephalosporins in Blood and Urine by HPLC

LI MU1, LIAO LIN-CHUAN1, YAN YOU-YI1, YANG LIN1, LIU XIAO-MIN1, DAI JIN2, MA XIAO-NA1, SHENG XIN1, HUANG HONG1, SU GUI1 (1. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041, CHINA; 2. SCHOOL OF PHARMACY, CHENGDU M   

  • Online:2009-12-25 Published:2009-12-28

摘要： 目的建立检测血液和尿液中头孢唑啉、头孢拉定、头孢哌酮和头孢噻肟的高效液相色谱法,并考察方法的适用性。方法以乙酰苯胺为内标,血液和尿液用乙腈直接沉淀蛋白,采用Agilent Zorbax SB-Aq(250mm×4.6mm,5μm)色谱柱,流动相选用水(含0.12%三乙胺和0.12%乙酸)和乙腈的混合溶剂梯度洗脱,流速1mL/min,检测波长254nm。结果4种头孢菌素分离完全,工作曲线相关性均良好(r>0.9990),检出限为0.01μg/mL,回收率均不低于81.2%。结论所建立的方法对血液和尿液中以上4种头孢菌素的检测快速、简便、准确,适用于实际检案中头孢菌素类药物的分析。

关键词: 法医毒理学, 头孢菌素类, 血液, 尿液, 高效液相色谱法

Abstract: Objective To establish a new high performance liquid chromatography(HPLC) method for determining the concentration of cefazolin, cefradine, cefoperazone and cefotaxime in blood and urine, as well as to investigate its applicability. Methods Protein in blood and urine was precipitated directly by acetonitrile with acetanilide was used as the internal standard using Agilent Zorbax SB-Aq column(250 mm× 4.6 mm,5 μm). The mixed solvents of water(triethylamine 0.12%, acetic acid 0.12%) and acetonitrile were used as the mobile phase to separate cephalosporins using gradient elution method at 1 mL/min (flow rate) and 254 nm(detection wavelength). Results The working curve of four cephalosporins showed a good cor- relation (r=0.999 3), with the detection limit up to 0.01 μg/mL. The recovery rate was more than 81.2%. Conclusion This method is fast, easy and accurate. It is suitable for biological analysis of the 4 cephalosporins of the blood and urine in practical cases.

Key words: forensic toxicology, cephalosporins, blood, urine, high-performance liquid chromatography (HPLC)