法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 440-442.

• 论文 • 上一篇    下一篇

差异提取试剂盒在混合斑DNA提取中的应用

吴丹;曹禹;许炎;何柏芳;毕钢;周怀谷;   

  1. 上海市公安局法医物证学现场应用技术公安部重点实验室上海市现场物证重点实验室;爱普拜斯应用生物系统贸易有限公司;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Validation of Differential Extraction Kit in Forensic Sexual Assault Cases

WU DAN1, CAO YU1, XU YAN1, HE BAI-FANG2, BI GANG1, ZHOU HUAI-GU1 (1. KEY LABORATORY OF FORENSIC EVIDENCE AND SCENE TECHNOLOGY, SHANGHAI KEY LABORATORY OF CRIME SCENE EVIDENCE, PUBLIC SECURITY BUREAU OF SHANGHAI, SHANGHAI 200083, CHINA; 2. APPLIED BIOSYSTE   

  • Online:2009-12-25 Published:2009-12-28

摘要： 目的评估差异提取试剂盒对混合斑样本中的精子和上皮细胞DNA分离提取的有效性。方法采用差异提取试剂盒,选择性裂解精细胞和上皮细胞,结合磁珠法分别对人为控制条件下制备的模拟混合样本和案件中的混合斑检材进行精细胞DNA和上皮细胞DNA的分离提取。对所提取的DNA进行定量分析和STR分型。结果该试剂盒能从精子和上皮细胞不同比例的混合斑中提取出高纯度的精细胞和上皮细胞DNA。结论该差异提取试剂盒适用于性侵害案件中混合斑检材的DNA提取。

关键词: 法医遗传学, 试剂盒, 诊断, 性犯罪, 差异提取, 混合斑

Abstract: Objective To evaluate the validity of Differential Extraction Kit in isolating spermatozoa and epithelial cell DNA from mixture samples. Methods Selective lysis of spermatid and epithelial cells combined with paramagnetic particle method were applied to extract the DNA from the mock samples under controlled conditions and forensic case samples, and template DNA were analyzed by STR genotype method. Results This Differential Extraction Kit is efficient to obtain high quality spermatid and epithelial cell DNA from the mixture samples with different proportion of sperm to epithelial cell. Conclusion The Differential Extraction Kit can be applied in DNA extraction for mixed stain from forensic sexual assault samples.

Key words: forensic genetics, reagent kits, diagnostic, sex offenses, differential extraction, mixed stain