法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (1): 33-36.DOI: 10.3969/j.issn.1004-5619.2018.01.007

• 论著 • 上一篇    下一篇

示指与接触物菌群ERIC-PCR指纹图谱比对

刘耘汀1，2，孙大明1，施少培2，杨  旭2   

  1. 1. 华东政法大学，上海 200043； 2. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台，上海 200063
  • 发布日期:2018-02-25 出版日期:2018-02-28
  • 通讯作者: 杨旭，男，高级工程师（正高级），主要从事文检、痕迹等物证技术方向研究；E-mail：yangx@ 施少培，男，高级工程师（正高级），主要从事文检、电子数据司法鉴定研究；E-mail：shisp@ssfjd.cn
  • 作者简介:刘耘汀（1994—），女，硕士研究生，主要从事刑事技术鉴定及研究；E-mail：yvonneliu1994@sina.com
  • 基金资助:

    国家社会科学基金资助项目（17BFX063）；上海市法医学重点实验室资助项目（17DZ2273200）；上海市司法鉴定专业技术服务平台资助项目（16DZ2290900）

Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants

LIU Yun-ting1,2, SUN Da-ming1, SHI Shao-pei2, YANG Xu2   

  1. 1. East China University of Political Science and Law, Shanghai 200043, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
  • Online:2018-02-25 Published:2018-02-28

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摘要： 目的 探讨示指菌群和接触物（手机触摸屏、个人办公桌桌面）表面菌群之间的关联性。 方法 采集10名志愿者的示指、手机触摸屏、个人办公桌桌面的菌群，通过宏基因组PCR扩增技术建立肠道细菌基因间重复序列（enterobacterial repetitive intergenic consensus，ERIC）-PCR指纹图谱。 结果 7名志愿者的示指与手机触摸屏菌群ERIC-PCR指纹图谱对应吻合，且各志愿者个体之间ERIC-PCR指纹图谱互不相同；3名志愿者的示指菌群与个人办公桌桌面菌群ERIC-PCR指纹图谱对应吻合，其余7名志愿者的示指菌群与个人办公桌桌面菌群ERIC-PCR指纹图谱不完全吻合，但分别存在大小一致的条带。 结论 示指所携带的细菌菌群具有一定个体特异性，示指接触手机触摸屏、办公桌桌面后残留下来的菌群，可作为潜在的法医学鉴定的新型生物样本。

关键词: 法医遗传学, 聚合酶链反应, DNA指纹法, 肠道细菌基因间重复序列, 指纹图谱

Abstract: Objective To explore the bacteria relevance between index fingers and contactant’ surfaces （mobile phone touch screen and desktop of personal office table）. Methods Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus （ERIC）-PCR fingerprint was established by PCR amplification technique of metagenome. Results There were 7 volunteers’ ERIC-PCR fingerprints of index fingers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers’ ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers’ ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table, but had at least one similar band for both. Conclusion The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.

Key words: forensic genetics, polymerase chain reaction, DNA fingerprinting, enterobacterial repetitive intergenic consensus, finger print

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