法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (2): 68-69.

• 论文 • 上一篇    下一篇

大鼠死后组织细胞DNA含量变化与死亡时间的相关性研究

刘志萍,陈新,沈忆文,赵子琴,顾云菊   

  1. 复旦大学上海医学院法医学系,上海市刑事科学技术研究所,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系,复旦大学上海医学院法医学系 上海200032 ,上海200083 ,上海200032 ,上海200032 ,上海200032
  • 发布日期:2004-04-25 出版日期:2004-04-28

Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat

LIU ZHI-PING1, CHEN XIN2, SHEN YI-WEN1, ET AL. (DEPARTMENT OF FORENSIC MEDICINE,MEDICAL CENTER OF FUDAN UNIVERSITY, SHANGHAI 200032, CHINA)   

  • Online:2004-04-25 Published:2004-04-28

摘要： 目的应用流式细胞术研究大鼠死后组织细胞DNA含量变化与死亡时间的相关性。方法SD大鼠断颈处死后于不同时间段取心、肝、脾、肾器官组织,经胰蛋白酶消化等处理后制成单细胞悬液,应用流式细胞术,在620nm波长处检测各组不同器官组织细胞DNA含量,观察其变化规律。结果大鼠各器官组织细胞DNA含量随死亡时间延长均呈下降趋势。其中以脾组织细胞DNA含量变化趋势与死亡时间最具相关性,肝、肾次之,而心最差;死后48h,各器官组织细胞仅存微量完整的细胞核DNA。结论机体死亡后,各器官组织细胞核DNA含量随死亡时间的延长逐渐减少,具有一定的变化规律,可应用组织细胞DNA含量变化来推断死亡时间。

关键词: 死亡时间, DNA含量, Feulgen染色, 流式细胞术

Abstract: Objective To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells. Methods After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats′viscera were detected by flow cytometry after stained by fluorescence , and also inspect the amount of DNA in different periods to find out the law of its variation. Results It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen. Conclusion The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI.

Key words: postmortem interval, DNA degrade, flow cytometry