法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 68-72.

• 论文 • 上一篇    下一篇

嗜尸性蝇类线粒体DNA分子标记检测的研究进展

蔡继峰,廖志钢   

  1. 四川大学华西基础与法医学院病理教研室,四川大学华西基础与法医学院病理教研室 四川成都610041 ,四川成都610041
  • 发布日期:2005-02-25 出版日期:2005-02-28

The Study of the Sequence of Molecular Markers of Mitochondrial DNA of Sarcosaphagous Flies

CAI JI-FENG, YING BIN-WU, TAO TAO, ET AL. (FORENSIC PATHOLOGY DEPARTMENT, SCHOOL OF LEGAL MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041,CHINA)   

  • Online:2005-02-25 Published:2005-02-28

被引次数

27

摘要： 嗜尸性蝇类种属鉴定是利用昆虫进行检案的重要一步,常常对案件的侦破起到关键作用。传统上,仅依据其形态学特征来判断嗜尸性蝇类的种属。由于其形态结构复杂和种间形态差异微小等特点,尤其在其幼期很难鉴别其种属。利用线粒体DNA(mtDNA)上细胞色素氧化酶辅酶Ⅰ和Ⅱ(COⅠ和COⅡ)的序列对嗜尸性蝇类进行种属鉴定,是近几年来发展起来的新技术,该检测方法能有效地将嗜尸性蝇类鉴定到属种的水平,目前国内尚无这方面的报道,本文对国外这方面工作的进展作一综述。

关键词: 嗜尸性苍蝇, 种属, 线粒体DNA, 分子标记, 细胞色素氧化酶辅酶Ⅰ, 细胞色素氧化酶辅酶Ⅱ

Abstract: Identifying sarcosaphagous flies specimens is an important first step in a forensic-entomological analysis. It is traditionally performed using morphological features of the Sarcosaphagous Flies. However, Morphological identification may be complicated by the numerical diversity of species and physical similarity between different species, particularly in immature stages. The sequences focused on some sections of the cytochrome oxidase Ⅰ and Ⅱ (COⅠ and COⅡ) encoding region of mtDNA could be as the prospective basis of a diagnostic technique. By Analysis of these sequences, forensic doctors can reveal abundant phylogenetic informative nucleotide substitutions that could effectively identify Sarcosaphagous flies to species group. It was not reported in our country before and was reviewed in this article now.

Key words: sarcosaphagous flies species, mitochondrial DNA, molecular marker COⅠ, molecular marker COⅡ