法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (5): 330-332.

• 论文 • 上一篇    下一篇

应用AFLP检测大麻遗传多样性

郭佳;裴黎;彭建雄;翟红;张贵芹;揭琴;涂政;   

  1. 中国人民公安大学;公安部物证鉴定中心;中国农业大学;
  • 发布日期:2008-10-25 出版日期:2008-10-28

DNA Polymorphism Detection of Cannabis Using Amplified Fragment Length Polymorphism

GUO JIA1,PEI LI2,PENG JIAN-XIONG2,ZHAI HONG3,ZHANG GUI-QIN1,JIE QIN3,TU ZHENG2 (1. CHINESE PEOPLE′S PUBLIC SECURITY UNIVERSITY,BEIJING 100038,CHINA; 2. INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF PUBLIC SECURITY,P.R.C,BEIJING 100038,CHINA; 3. CHINA AGRICULT   

  • Online:2008-10-25 Published:2008-10-28

被引次数

20

摘要： 目的筛选对于大麻多态性好的AFLP引物标记来区分大麻品种。方法利用AFLP技术用55对引物组合对12个大麻地域品种进行初筛。结果选出5对多态性好的引物组合进行了遗传多样性研究。每对AFLP引物组合扩增出47～76条带,共获得285条带,其中多态性条带为99条以及10条品种特异带。结论AFLP对于大麻具有很高的分辨率,为今后深入地研究大麻植物遗传多样性奠定了很好的基础。

关键词: 法医植物学, 遗传多样性, 大麻, 扩增片段长度多态性

Abstract: Objective To screen the AFLP primers with good diversity to distinguish various species of Cannabis. Methods The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations. Results A total of 285 AFLP bands were obtained using five primer combinations with better diversity,among which 99 bands were polymorphic and 10 bands were special,with 47-76 bands amplified in each pair of primers. Conclusion AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.

Key words: forensic biology, genetic diversity, Cannabis, AFLP