法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (2): 85-87.

• 论文 • 上一篇    下一篇

大鼠挫伤肺组织中水通道蛋白1表达变化

袁军;陈阳;程利宝;   

  1. 皖南医学院法医病理学教研室;
  • 发布日期:2009-04-25 出版日期:2009-04-28

Changes of Aquaporins 1 Expression in the Contused Lung of Rats

YUAN JUN, CHEN YANG, CHENG LI-BAO (DEPARTMENT OF FORENSIC PATHOLOGY, WANNAN MEDICAL COLLEGE, WUHU 241002, CHINA)   

  • Online:2009-04-25 Published:2009-04-28

被引次数

15

摘要： 目的探讨水通道蛋白1(aquaproin 1,AQP1)在大鼠挫伤肺组织中的表达变化及其与肺水肿的关系。方法将SD大鼠随机分为实验组和对照组,制备肺挫伤模型,用免疫组织化学方法检测肺组织中AQP1的分布和表达变化。结果肺挫伤后1和3h肺组织出现水肿、出血,炎性细胞浸润,5h炎性反应进一步加重。AQP1表达在挫伤后1、3和5h均明显高于对照组(P<0.01),随着伤后时间延长,水肿加重,AQP1持续上升。正常AQP1主要分布在支气管和肺泡壁的毛细血管内皮细胞及间质细胞,挫伤后肺AQP1的表达部位未发生改变,但积分光密度值(intergrated optical density,IOD)差异有统计学意义(P<0.01)。结论挫伤肺组织可能存在AQPs的基因表达调控失衡,导致大量水的异常跨膜转运以及在肺组织中的异常聚集,这可能是挫伤肺组织水肿形成原因之一。

关键词: 法医病理学, 水通道蛋白质1, 挫伤, 肺水肿, 大鼠

Abstract: Objective To investigate the expression changes of aquaporins 1(AQP1) in contused lung tissue of rats and its relationship with pulmonary edema. Methods SD rats were randomly divided into experimental and control groups. The pulmonary contusion models were then prepared. The expression and distribution of AQP1 in lung tissue of the rats were detected by immunohistochemistry. Results The lung tissue showed edema, hemorrhage, inflammatory cell infiltration 1 h, 3 h after pulmonary contusion, and the inflammatory response aggravated after 5 h. AQP1 expression at 1 h, 3 h and 5 h in the contusion group were significantly higher than that of the control group(P<0.01). The expression of AQP1 continued to increase with time and aggravation of edema compared to the control group. AQP1 was mainly distributed in the capillary endothelial cells and interstitial cells of the bronchial and alveolar walls. Although there were no observed changes in AQP1 expression location in contused lung tissue, the intergrated optical density(IOD) showed significant statistical difference(P<0.01). Conclusion There might exist an dysregulation of AQPs gene expression in contused lung tissue, leading to a large number of abnormal transmembrane water transportation and abnormal water accumulation, which may be one of the reasons for pulmonary edema in contused lung tissue.

Key words: forensic pathology, aquaporins 1, contusions, pulmonary edema, rats