法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (2): 123-126.

• 论文 • 上一篇    下一篇

LC-MS/MS同时测定人血浆中氯氮平、奥氮平和米氮平

王贤亲;潘晓军;林观样;向铮;王学宝;吴建章;林丹;   

  1. 温州医学院分析测试中心;温州医学院药学院;温州医学院附属第一医院;
  • 发布日期:2009-04-25 出版日期:2009-04-28

Simultaneous Determination of Clozapine, Olanzapine and Mirtazapine in Human Plasma by LC-MS/MS

WANG XIAN-QIN1, PAN XIAO-JUN2, LIN GUAN-YANG3, XIANG ZHENG2, WANG XUE-BAO1, WU JIAN-ZHANG2, LIN DAN1 (1. ANALYTICAL AND TESTING CENTER, WENZHOU MEDICAL COLLEGE, WENZHOU 325035, CHINA; 2. SCHOOL OF PHAR- MACY, WENZHOU MEDICAL COLLEGE, WENZHOU 325035, CHINA   

  • Online:2009-04-25 Published:2009-04-28

被引次数

8

摘要： 目的建立人血浆中氯氮平、奥氮平、米氮平的液相色谱-串联质谱(LC-MS/MS)检测方法。方法血浆中的氯氮平、奥氮平、米氮平用乙醚液-液萃取,采用Agilent Zorbax SB-C18柱(2.1mm×150mm,5μm),电喷雾离子源,正离子检测,多反应监测方式进行定量分析,卡马西平作为内标。结果氯氮平、奥氮平和米氮平的检测限为0.41～0.92ng/mL,质量浓度在10.0～2000.0ng/mL范围内线性良好(r≥0.9924),平均萃取回收率在65.7%～94.2%,日内、日间精密度RSD≤6%(n=5)。结论该方法专属性强、灵敏及准确,可以适用于法庭与临床毒物分析。

关键词: 法医毒理学, 液相色谱-串联质谱法, 氯氮平, 奥氮平, 米氮平, 血浆

Abstract: Objective To develop a method for determination of clozapine, olanzapine and mirtazapine in human plasma by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Methods Clozapine, olanzapine and mirtazapine were extracted from plasma samples by using diethyl ether and separated by Agilent Zorbax SB-C18 column(2.1 mm×150 mm, 5 μm). Electrospray ionization source was applied, positive ion mode was used to detect and multiple reaction monitoring mode was used to quantify clozapine, olanzapine and mirtazapine. Carbamazepine was the internal standard. Results The detection limits of clozapine, olanzapine and mirtazapine were within 0.41-0.92 ng/mL. The calibration curve in the concentration range of 10.0-2 000.0 ng/mL showed a good linear distribution (r≥0.992 4). The average extraction recoveries were within 65.7%-94.2%. Intra-day RSD and inter-day RSD were less than 6%(n=5). Conclusion This method seems to be quite specific, sensitive and accurate, and can be used to detect clozapine, olanzapine and mirtazapine in forensic and clinical analytic toxicology.

Key words: forensic toxicology, liquid chromatography-tandem mass spectrometry(LC-MS/MS), clozapine, olanzapine, mirtazapine, plasma