法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (5): 359-361.

• 论文 • 上一篇    下一篇

不同温度下血样中毒鼠强的稳定性

卜俊;刘伟;严慧;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;
  • 发布日期:2009-10-25 出版日期:2009-10-28

Stability of the Tetramine in Blood Samples under Different Temperatures

BU JUN,LIU WEI,YAN HUI(SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA)   

  • Online:2009-10-25 Published:2009-10-28

摘要： 目的探讨不同保存温度下血样中毒鼠强的稳定性。方法配制毒鼠强质量浓度为0.5μg/mL的血液样品,分别置于45℃、25℃和4℃环境中保存,在配制当天及存放3、12、18和39d用气相色谱-质谱联用法测定其质量浓度,运用SPSS统计软件对结果进行统计分析。结果45℃、25℃下血样存放3d毒鼠强质量浓度基本不变,存放4～12d毒鼠强质量浓度有明显下降,存放12d后毒鼠强质量浓度缓慢下降;4℃下血样存放12d毒鼠强质量浓度基本不变,存放13～18d毒鼠强质量浓度有明显下降,存放18d后毒鼠强样品质量浓度缓慢下降。结论血样中毒鼠强质量浓度在最初的3d内稳定,此后浓度下降。保存温度对血样中毒鼠强质量浓度有影响。

关键词: 法医毒理学, 毒鼠强, 稳定性, 血液样品

Abstract: Objective To study the stability of tetramine in blood samples under different environment tempera-tures.Methods The tetramine samples in 0.5 μg/mL concentration were stored in the environment temperature at 45 ℃,25 ℃ and 4 ℃.The tetramine concentration were measured by GC-MS in 1,3,12,18 and 39 days,respectively.And then the results of measurement were analyzed by SPSS software.Results At 45 ℃ and 25 ℃,the concentration of tetramine changed without distinctly in first 3 days and declined significantly from 4 days to 12 days.Thereafter it declined more slowly.At 4 ℃,the concentration of tetramine changed without distinctly in 12 days and declined significantly from 13 days to 18 days.Thereafter it declined more slowly.Conclusion The concentration of tetra mine in blood samples is stable in 3 days.Thereafter it declined gradually.Temperature also influences the concentration of tetra mine.

Key words: forensic toxicology, tetramine, stability, blood samples