法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (5): 506-508.DOI: 10.3969/j.issn.1004-5619.2017.05.012

• 论著 • 上一篇    下一篇

GC-MS分析尿液中甲卡西酮

刘冬娴，赵明明   

  1. 湖南警察学院刑事科学技术系，湖南 长沙 410138
  • 发布日期:2017-10-25 出版日期:2017-10-28
  • 作者简介:刘冬娴（1963—），女，教授，主要从事理化检验教学与科研；E-mail：liudongxian@aliyun.com
  • 基金资助:

    湖南省教育厅科学研究重点资助项目（15A057）

Determination of Methcathinone in Urine by GC-MS

LIU DONG-XIAN, ZHAO MING-MING   

  1. Department of Criminal Science and Technology, Hunan Police Academy, Changsha 410138, China
  • Online:2017-10-25 Published:2017-10-28

被引次数

4

摘要： 目的 建立尿液中甲卡西酮的气相色谱-质谱（gas chromatography-mass spectrometry，GC-MS）分析方法。 方法 在尿液中加入内标双苯戊二氨酯（SKF525A）和pH=9的缓冲溶液，用乙酸乙酯提取，提取液在50 ℃氮气流下挥干，残余物用甲醇溶解，用GC-MS分析。 结果 尿液中甲卡西酮在0.02~2.00 μg/mL质量浓度范围内线性关系良好，线性方程为y=0.301 9 x+0.018 9（r=0.999 2），检出限为0.01 μg/mL。尿液中甲卡西酮回收率为96.4%~99.2%，日内精密度为5.8%~7.6%，日间精密度为6.0%~8.1%。 结论 该方法操作简便、灵敏度高，可用于司法鉴定实践尿液样品中甲卡西酮的分析。

关键词: 法医毒理学, 气相色谱-质谱法, 尿液, 甲卡西酮

Abstract: Objective To establish a method for the analysis of methcathinone in urine by gas chromatography-mass spectrometry （GC-MS）. Methods Proadifen hydrochloride （internal standard） and buffer solution （pH=9） were added into the urine samples, and methcathinone was extracted by ethyl acetate. The extract was volatilized in 50 ℃ nitrogen gas flow and the remnant was dissolved by methanol and analysed by GC-MS. Results The methcathinone in urine showed a good linear relationship in the mass concentration range of 0.02-2.00 μg/mL. The linear equation was y=0.301 9 x+0.018 9 （r=0.999 2）, and the detection limit was 0.01 μg/mL. The recoveries of methcathinone in urine was 96.4%-99.2%, with the intra-day precision of 5.8%-7.6% and the inter-day precision of 6.0%-8.1%. Conclusion The method is convenient and sensitive, which can be applied to the forensic identification of methcathinone in urine.

Key words: forensic toxicology, gas chromatography-mass spectrometry, urine, methcathinone

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