Assessment on Application of 24 Y-STR Loci in Forensic Science
LI Min1,2, HUANG Lei3, WANG Xin-jie4, CHEN Yu-ling2,5, SHENG Xiang2,6, LI Ya-nan2,7, BAO Yun2,7, JIANG Lei2, ZHU Ru-xin2, XU Qian-nan1,2, ZHANG Jia-shuo2,6, LI Cheng-tao1,2, BIAN Ying-nan1,2
1. Department of Forensic Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China; 3. Shandong Provincial Public Security Department, Jinan 250001, China; 4. Weifang Public Security Bureau, Weifang 261061, China; 5. State Key Laboratory of Bioreactor Engineering, College of Biology Engineering, East China University of Science and Technology, Shanghai 200237, China; 6. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 7. Department of Forensic Medicine, Inner Mongolia Medical University, Hohhot 010030, China
Objective To select a Y-STR marker system with strong haplotype identification ability, appropriate mutation rate and high compatibility and to assess its forensic application. Methods The 24 Y-STR loci were tested by self-built fluorescent multiplex system, and the forensic assessment was conducted by 139 pairs of father-son samples collected in Jinan, Shandong province. Results Totally 176 alleles were identified among the 24 Y-STR loci in the sample of 139 unrelated individuals labeled with father, and the gene diversity （GD） distributed between 0.083 7 （DYS645）-0.966 9 （DYS385a/b）. According to the 24 Y-STR loci, 139 different haplotypes were detected from 139 unrelated male individuals labeled with father in Han population of Shandong province and with no shared haplotype observed. The overall haplotype diversity （HD） was 1 and the discrimination capacity （DC） was 1. A total of 5 one-step mutations events were observed among the 24 Y-STR loci in 139 pairs of father-son. The average mutation rate was 0.001 5 [95% CI （0.000 5, 0.003 5）]. Conclusion The system of 24 Y-STR loci shows a strong individual recognition ability and low mutation rate in the population in Jinan, Shandong province, and it has good application value in forensic science.