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法医学杂志 2018, Vol. 34 Issue (4) :375-378,383    DOI: 10.12116/j.issn.1004-5619.2018.04.006
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LC-MS/MS法快速测定头发中可卡因及其代谢物苯甲酰爱康宁
潘美如1,2,强火生3,沈保华2,严  慧2,向  平2
1. 沈阳药科大学,辽宁 沈阳 110016; 2. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台,上海 200063; 3. 广州万孚生物技术股份有限公司,广东 广州 510663
Rapid Determination of Cocaine and Its Metabolite Benzoylecgonine in Hair by LC-MS/MS
PAN Mei-ru1,2, QIANG Huo-sheng3, SHEN Bao-hua2, YAN Hui2, XIANG Ping2
1. Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China; 3. Guangzhou Wondfo Biotech Co. Ltd, Guangzhou 510663, China

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摘要 目的 建立头发中可卡因(cocaine)及其代谢物苯甲酰爱康宁(benzoylecgonine)的液相色谱-串联质谱(LC-MS/MS)快速测定方法。 方法 去污处理后的头发中加入氘代内标(可卡因-D3和苯甲酰爱康宁-D8),经甲醇超声提取后用Restek Allure PFP丙基柱分离,采用多反应监测模式同时测定可卡因和苯甲酰爱康宁。 结果 头发中可卡因和苯甲酰爱康宁在0.02~10.00 ng/mg质量分数范围内线性关系良好,检出限均为0.01 ng/mg。 结论 本研究所建方法样品前处理简单、快速、选择性好,适用于头发中可卡因及代谢物苯甲酰爱康宁的检测。
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潘美如
强火生
沈保华
关键词法医毒理学   液相色谱-串联质谱   可卡因   苯甲酰爱康宁   头发     
Abstract: Objective To establish a rapid determination method with LC-MS/MS for cocaine and its metabolite benzoylecgonine in hair. Methods Deuterated internal standards (cocaine-D3 and benzoylecgonine-D8) were added to the decontaminated hair. After the extraction by ultrasonication with methanol, the compounds were separated by the Restek Allure PFP propyl column, and cocaine and benzoylecgonine were simultaneously analysed in multiple reaction monitoring mode. Results The cocaine and benzoylecgonine in hair showed a good linearity in the range of mass fraction between 0.02 and 10.00 ng/mg with the limits of detection of 0.01 ng/mg. Conclusion The developed method is simple and rapid with a good selectivity, which is suitable for the determination of cocaine and its metabolite benzoylecgonine in hair.
Keywordsforensic toxicology   LC-MS/MS   cocaine   benzoylecgonine   hair     
Fund:“十三五”国家重点研发计划资助项目(2016YFC0800704);中央级公益性科研院所专项资金资助项目(GY2017D-1);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)
Corresponding Authors: 向平,女,研究员,主要从事法医毒物学研究和鉴定;E-mail:xiangp@ssfjd.cn   
About author: 潘美如(1994—),女,硕士研究生,主要从事药物分析研究;E-mail:13818940774@163.com
引用本文:   
潘美如,强火生,沈保华,等.LC-MS/MS法快速测定头发中可卡因及其代谢物苯甲酰爱康宁[J]  法医学杂志, 2018,34(4): 375-378,383
PAN Mei-ru, QIANG Huo-sheng, SHEN Bao-hua,et al..Rapid Determination of Cocaine and Its Metabolite Benzoylecgonine in Hair by LC-MS/MS[J]  法医学杂志, 2018,34(4): 375-378,383
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