Application of High-throughput Sequencing in Researches of Cadaveric Microorganisms and Postmortem Interval Estimation
FU Xiao-liang1, GUO Juan-juan1, LIU Zhuo-ying1, SHEN Xiao2, CAI Ji-feng1
1. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China; 2. Public Security Department of Lhasa, Liuwu Xintong Railway Station of Lhasa, Lhasa 850000, China
Necrobiome is the main factor causing the cadaver decomposition. Studying the microbial succession during decomposition is one of the main tasks of forensic microbiology. The interactive relationships among cadaver, environment and microorganisms are complicated. The microbial succession study relies on macroscopic monitoring of community composition and the diversity change in each decomposition stage. With the maturity and development of high-throughput sequencing （HTS）, the structure and diversity of microbial communities in different environments have been successively revealed. A new breakthrough to explore the cadaveric microorganisms has been opened as well. It has become the research hotspots in forensic microbiology to reveal the microbial succession in the process of cadaver decomposition and to interpret the essence of various decomposition phenomena by using HTS, which can provide a new reference for postmortem interval （PMI） estimation. The present paper reviews studies on PMI estimation by using cadaveric microorganism. Problems and application prospects of forensic microbiology studies are discussed on the basis of the current application of HTS technology in the exploration of microbial succession.