法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (6): 635-639.DOI: 10.12116/j.issn.1004-5619.2018.06.012

• 论著 • 上一篇    下一篇

大鼠视神经挫伤致视网膜硫化氢合成酶表达变化

曾铭伟1，2，王  涛1，费成平1，2，邹彩霞1，刘夷嫦1，谷振勇1，2   

  1. 1. 南通大学医学院法医学系，江苏 南通 226001； 2. 南通大学附属医院司法鉴定所，江苏 南通 226001
  • 发布日期:2018-12-25 出版日期:2018-12-28
  • 通讯作者: 谷振勇，男，博士，教授，博士研究生导师，主要从事法医病理学研究；E-mail：zygusz@126.com
  • 作者简介:曾铭伟（1982—），男，硕士，主检法医师，主要从事法医临床学鉴定和眼外伤基础研究；E-mail：sfjdzmw@163.com
  • 基金资助:
    上海市法医学重点实验室开放课题资助项目（1308KF）；江苏省高校优势学科建设工程资助项目（PAPD）

Optic Nerve Crush Induces Changes of Hydrogen Sulfide Synthases Expression in the Rat Retina

ZENG Ming-wei1,2, WANG Tao1, FEI Cheng-ping1,2, ZOU Cai-xia1, LIU Yi-chang1, GU Zhen-yong1,2   

  1. 1. Department of Forensic Medicine, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China; 2. Institute of Judical Expertise, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
  • Online:2018-12-25 Published:2018-12-28

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摘要： 目的 探讨大鼠视神经挫伤后视网膜硫化氢合成酶胱硫醚γ-裂解酶（cystathionine γ-lyase，CSE）、胱硫醚β-合成酶（cystathionine β-synthase，CBS）和3-巯基丙酮酸硫基转移酶（3-mercaptopyruvate sulfurtransferase，3-MST）的表达变化。 方法 建立大鼠视神经挫伤模型。将大鼠分为正常对照组、视神经挫伤组和假手术组。观察大鼠视网膜的组织病理学变化，检测视网膜神经节细胞数量和视网膜内侧部分厚度。用实时荧光定量PCR技术检测CBS、CSE和3-MST的mRNA表达变化。 结果 正常对照组大鼠视网膜组织结构正常，假手术组仅有轻微变化。与假手术组比较，视神经挫伤后各组大鼠视网膜出现明显损伤，视网膜神经节细胞数量呈时间依赖性减少且视网膜内侧部分厚度呈进行性变薄。正常对照组大鼠视网膜存在CSE、CBS和3-MST mRNA的基础表达。与正常对照组比较，假手术组CSE、CBS和3-MST的mRNA表达无明显变化。与假手术组比较，CBS mRNA表达在视神经挫伤后3 d、7 d和14 d呈时间依赖性上调；CSE mRNA表达在伤后7 d达到峰值，14 d时有所下降；3-MST mRNA表达在视神经挫伤后3 d和7 d有上调的趋势，14 d时有明显上调。 结论 大鼠视神经挫伤后视网膜硫化氢合成酶CBS、CSE和3-MST表达上调，这可能引起视网膜局部内源性硫化氢生成增多并产生代偿性保护效应。

关键词: 法医病理学, 视神经损伤, 视网膜, 硫化氢合成酶, 胱硫醚&gamma, -裂解酶, 胱硫醚&beta, -合成酶, 3-巯基丙酮酸硫基转移酶, 大鼠

Abstract: Objective To investigate the expression changes of the hydrogen sulfide synthases cystathionine γ-lyase （CSE）, cystathionine β-synthase （CBS）, and 3-mercaptopyruvate sulfurtransferase （3-MST）, after optic nerve crush （ONC） in rat the retina. Methods The rat model of ONC was established. Rats were divided into normal control, ONC, and sham control groups. Histopathologic changes in retina, the number of retinal ganglion cells （RGC） and retinal thickness of inner part （RTIP） were measured. The changes of CSE, CBS and 3-MST mRNA expression were detected with quantitative real-time PCR. Results The retinal histostructure was normal in normal controls and with minor changes in sham controls, respectively. Compared with sham group, significant retina damages were found in the ONC group： a time-dependent reduction of RGC number and RTIP. Expressions of CSE, CBS and 3-MST mRNA in rat retina were detected in normal control. Compared with normal controls, the expressions of CSE, CBS and 3-MST mRNA did not show any significant changes in the sham controls. Compared with sham controls, CBS mRNA expressions showed a time-dependent increase at 3 d, 7 d and 14 d after crush in the ONC group; CSE mRNA expressions increased to the peak at 3 d and then slightly reduced at 14 d after crush; 3-MST mRNA expressions showed the trend of increase at 3 d and 7 d and then enhanced remarkably at 14 d after crush. Conclusion Hydrogen sulfide synthases CSE, CBS and 3-MST expressions were up-regulated in rat retina following ONC, which may cause an increase in local endogenous hydrogen sulfide production in the retina and a compensatory protective effect.

Key words: forensic pathology, optic nerve injuries, retina, hydrogen sulfide synthase, cystathionine γ-lyase, cystathionine β-synthase, 3-mercaptopyruvate sulfurtransferase, rats