Pathway of Diatoms Enter Experimental Rabbits through the Lymphatic System of the Digestive Tract

doi:10.12116/j.issn.1004-5619.2021.410607

Abstract

Abstract: Objective

To study whether diatoms can enter the body through the lymphatic system of the digestive tract.

Methods

Twenty experimental rabbits were divided into the test group and the control group randomly, and intragastric administration was performed with 20 mL water sample from the Pearl River and 20 mL ultrapure water, respectively. After 30 min, lymph, lungs， livers and kidneys were extracted for the diatom test. The concentration, size and type of diatoms were recorded.

Results

The concentration of diatoms of the test group was higher than that of the control group （P<0.05）. In the test group, Stephanodiscus, Coscinodiscus, Cyclotella, Melosira， Nitzschia， Synedra， Cymbella, and Navicula were detected; in the control group， Stephanodiscus, Coscinodiscus and Cyclotella were detected. The long diameter and the short diameter of diatoms of the test group were higher than those of the control group （P<0.05）. In the test group, 1-2 diatoms were detected in 3 lung samples and 2 liver samples, which were Stephanodiscus or Cyclotella, and no diatoms were detected in the kidney samples; in the control group, 1-2 diatoms were detected in 2 lung samples and 3 liver samples, which were Stephanodiscus or Coscinodiscus, and no diatoms were detected in the kidney samples.

Conclusion

Diatoms can enter the body through the lymphatic fluid, which is one of the reasons for the presence of diatoms in tissues and organs of non-drowning cadavers.

Key words: forensic pathology, drowning, diatom, digestive tract, lymph, gavage model

CLC Number:

DF795.1

Yu-kun DU, Jing-jian LIU, Xiao-dong KANG, Zhong-hao YU, Dong-yun ZHENG, He SHI, Qu-yi XU, Jian-jun REN, Chao LIU, Jian ZHAO. Pathway of Diatoms Enter Experimental Rabbits through the Lymphatic System of the Digestive Tract[J]. Journal of Forensic Medicine, 2022, 38(1): 67-70.

Figures/Tables 4

References 19

1	AUER A， MOTTONEN M. Diatoms and drowning[J]. Z Rechtsmed，1988，101（2）：87-98. doi：10.1007/BF00200290 .
2	PIETTE M H A， DE LETTER E A. Drowning： Still a difficult autopsy diagnosis[J]. Forensic Sci Int，2006，163（1）：1-9. doi：10.1016/j.forsciint.2004.10.027 .
3	LUNETTA， PENTTIL， LLFORS G H. Scanning and transmission electron microscopical evidence of the capacity of diatoms to penetrate the alveolo-capillary barrier in drowning[J]. Int J Legal Med，1998，111（5）：229-237. doi：10.1007/s004140050159 .
4	HÜRLIMANN J， FEER P， ELBER F， et al. Diatom detection in the diagnosis of death by drowning[J]. Int J Legal Med，2000，114（1/2）：6-14. doi：10.1007/s004149900122 .
5	刘超. 溺死法医诊断学[M]. 广州：中山大学出版社，2018.
	LIU C. Diagnosis of drowning[M]. Guangzhou： Sun Yat-sen University Press，2018.
6	FOGED N. Diatoms and drowning - Once more[J]. Forensic Sci Int，1983，21（2）：153-159. doi：10.1016/0379-0738（83）90104-4 .
7	MUELLER B. On the problem of the occurrence of diatoms in the organs of cadavers not having lain in the water[J]. Dtsch Z Gesamte Gerichtl Med，1963，54：267-272.
8	PORAWSKI R. Investigations on the occurrence of diatoms in organs in death from various causes[J]. J Forensic Med，1966，13（4）：134-137.
9	SCHELLMANN B， SPERL W. Detection of diatoms in bone marrow （femur） of nondrowned[J]. Z Rechtsmed，1979，83（4）：319-324. doi：10.1007/BF01882178 .
10	SHEN X， LIU Y， XIAO C，et al. Analysis of false-positive results of diatom test in the diagnosis of drowning-would not be an impediment[J]. Int J Legal Med，2019，133（6）：1819-1824. doi：10.1007/s00414-019-02021-4 .
11	张平萍. 消化道和呼吸道屏障对进入体循环硅藻大小的影响研究[D]. 昆明：昆明医科大学，2020.
	ZHANG P P. Effects of digestive and respiratory barriers on the size of diatoms entering systemic circulation[D]. Kunming： Kunming Medical University，2020.
12	陈静. 毛细淋巴管—组织间隙的液体传质渗流模型[D]. 太原：太原理工大学，2007.
	CHEN J. Study on fluid mass transfer patten in lymphatic capillary and tissue space[D]. Taiyuan： Taiyuan University of Technology，2007.
13	IONAC M. One technique, two approaches, and results： Thoracic duct cannulation in small laboratory animals[J]. Microsurg，2003，23（3）：239-245. doi：10.1002/micr.10136 .
14	李逊斗. 食物中能够进入淋巴液的蛋白尿蛋白质组影响因素及肾癌标志物的研究[D]. 北京：北京协和医学院，2014.
	LI X D. Proteomic strategy to investigate food proteins that can enter lymph， effects of common factors on urinary proteome and urinary biomarkers for ccRCC[D]. Beijing： Peking Union Medical College，2014.
15	ZHAO J， LIU C， HU S，et al. Microwave digestion - vacuum filtration - automated scanning electron microscopy as a sensitive method for forensic diatom test[J]. Int J Legal Med，2013，127（2）：459-463. doi：10.1007/s00414-012-0756-9 .
16	EN L Y， JAYAPRAKASH P T. Prevalence of diatom frustules in non-vegetarian foodstuffs and its implications in interpreting identification of diatom frustules in drowning cases[J]. Forensic Sci Int，2007，170（1）：1-7. doi：10.1016/j.forsciint.2006.08.020 .
17	KRSTIC S， DUMA A， JANEVSKA B， et al. Diatoms in forensic expertise of drowning -- A macedonian experience[J]. Forensic Sci Int，2002，127（3）：198-203. doi：10.1016/s0379-0738（02）00125-1 .
18	成令忠，钟翠平，蔡文琴. 现代组织学[M]. 上海：上海科学技术文献出版社，2003.
	CHENG L Z， ZHONG C P， CAI W Q. Contemporary histology[M]. Shanghai： Shanghai Scientific and Technological Literature Publication House，2003.
19	曹珊，王晶桐，刘玉兰. 胃肠道黏膜屏障与自身免疫性疾病[J].胃肠病学和肝病学杂志，2007，16（2）：198-200. doi：10.3969/j.issn.1006-5709.2007.02.034 .
	CAO S， WANG J T， LIU Y L. Gastrointestinal mucosa barrier and autoimmune disease[J]. Wei-changbingxue He Ganbingxue Zazhi，2007，16（2）：198-200.

组别	中心纲	羽纹纲
对照	100.0	0
实验	74.4^1）	25.6^1）
水样	88.7	11.3

组别	中心纲	羽纹纲
对照	100.0	0
实验	74.4^1）	25.6^1）
水样	88.7	11.3

组别	样本数/例	硅藻		中心纲直径	羽纹纲
组别	样本数/例	长径	短径	中心纲直径	长径	短径
对照	10	6.88（5.43~8.03）	6.88（5.43~8.03）	6.88（5.43~8.03）	-	-
实验	10	12.6（7.00~30.00）	6.79（4.30~13.20）	9.84（5.94~21.33）	31.4（14.52~56.80）	4.00（3.26~5.00）
水样	3	8.36（5.37~16.00）	6.39（4.00~10.50）	7.29（4.96~14.60）	27.8（9.79~38.40）	4.00（3.08~5.00）

组别	样本数/例	硅藻		中心纲直径	羽纹纲
组别	样本数/例	长径	短径	中心纲直径	长径	短径
对照	10	6.88（5.43~8.03）	6.88（5.43~8.03）	6.88（5.43~8.03）	-	-
实验	10	12.6（7.00~30.00）	6.79（4.30~13.20）	9.84（5.94~21.33）	31.4（14.52~56.80）	4.00（3.26~5.00）
水样	3	8.36（5.37~16.00）	6.39（4.00~10.50）	7.29（4.96~14.60）	27.8（9.79~38.40）	4.00（3.08~5.00）

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