Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy

doi:10.12116/j.issn.1004-5619.2023.430317

Abstract

Abstract:

Objective Fourier transform infrared spectroscopy （FTIR） was used to analyze myocardial infarction tissues at different stages of pathological change to achieve the forensic pathology diagnosis of acute and old myocardial infarction. Methods FTIR spectra data of early ischemic myocardium, necrotic myocardium, and myocardial fibrous tissue in the left ventricular anterior wall of the sudden death group of atherosclerotic heart disease and the myocardium of the normal control group were collected using hematoxylin-eosin （HE） and immunohistochemistry （IHC） staining as a reference, and the data were analyzed using multivariate statistical analysis. Results The mean normalized spectra of control myocardium, early ischemic myocardium and necrotic myocardium were relatively similar, but the mean second derivative spectra were significantly different. The peak intensity of secondary structure of proteins in early ischemic myocardium was significantly higher than in other types of myocardium, and the peak intensity of the α-helix in necrotic myocardium was the lowest. The peaks of amide Ⅰ and amide Ⅱ in the mean normalized spectra of myocardial fibrous tissue significantly shifted towards higher wave numbers, the peak intensities of amide Ⅱ and amide Ⅲ were higher than those of other types of myocardium, and the peak intensities at 1 338, 1 284, 1 238 and 1 204 cm^-1 in the mean second derivative spectra were significantly enhanced. Principal component analysis （PCA） and partial least square-discriminant analysis （PLS-DA） showed that FTIR could distinguish different types of myocardium. Conclusion FTIR technique has the potential to diagnose acute and old myocardial infarction, and provides a new basis for the analysis of the causes of sudden cardiac death.

Key words: forensic pathology, Fourier transform infrared spectroscopy （FTIR）, acute myocardial infarction, old myocardial infarction, principal component analysis, partial least square-discriminant analysis

CLC Number:

Tian TIAN, Xin-biao LIAO, Fu ZHANG, Kai-fei DENG, Ji ZHANG, Ping HUANG, Yi-jiu CHEN, Jian-hua ZHANG. Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy[J]. Journal of Forensic Medicine, 2023, 39(6): 535-541.

Figures/Tables 6

Fig. 1 Pathological changes of the control myocardium and early ischemic myocardium

Fig. 2 Pathological changes of the necrotic myocardium and myocardial fibrous tissue （HE staining）

Fig. 3 The mean normalized spectra and the mean second derivative spectra of different types of myocardium

Fig. 4 PCA scores plot， PC2 loading plot and PLS-DA scores plot of the second derivative spectra ofdifferent types of myocardium

Fig. 5 PCA scores plot， PC2 loading plot and PLS-DA scores plot of the second derivative spectra ofcontrol myocardium， early ischemic myocardium and necrotic myocardium

Fig. 6 PCA scores plots and PLS-DA scores plots for two-by-two subgroups of the second derivative spectra ofcontrol myocardium， early ischemic myocardium and necrotic myocardium

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