Abstract: Objective To investigate polymorphism distribution of the 5 Y-SNP loci in Jinan Han population, and evaluate their potential in forensic application. Methods Genotyping of 5 Y-SNP loci（M89, M9, M122, M134, M95） were executed in the sample of 103 unrelated Chinese male individuals in Jinan Han population by using fragment length discrepant allele specific PCR（FLDAS-PCR）. Results In 5 Y-SNP loci, genetic polymorphism were identified in Jinan Han population, and the ranges of gene diversity（GD） were 0.093 3-0.491 2. Twenty different haplotypes were observed and the haplotypes diversity（HD） was 0.867 9. Six different haplogroups were detected according to international association of Y chromosome nomenclature. Conclusion Five Y-SNP loci and their haplogroups in Jinan Han population are highly polymorphic, which can provide more information for the genetic structure analysis and forensic genetics research in the region.

Key words: forensic genetics, polymorphism, single nucleotide, Y chromosome, Han nationality