›› 2011, Vol. 27 ›› Issue (6): 444-446.DOI: 10.3969/j.issn.1004-5619.2011.06.012

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Rapid DNA Identification Using 6+1 STR Kit and EX-Q20 Electrophoresis

PING YUAN1,2, ZHOU HUAI-GU1, XU YAN1, XIA ZI-FANG3, ZHENG WEI-GUO3   

  1. (1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. School of Life Sciences, Fudan University, Shanghai 200433, China; 3. AGCU Biotechnology Company, Wuxi 214174, China)
  • Online:2011-12-25 Published:2011-12-28

Abstract: Objective To establish a rapid STR genotyping method for individual identification. Methods Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and SinofilerTM kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared. Results 6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time. Conclusion 6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.

Key words: forensic genetics, STR, individual identification, rapid identification

CLC Number: