Abstract: Objective To perform the validation and analysis of forensic parameters of Goldeneye®DNA ID 26Y system. Methods Based on the validation rules of Scientific Working Group on DNA Analysis Methods （SWGDAM）, the kit was assessed from several parts, as test of PCR system, reproducibility, accuracy, and sensitivity, etc. And Y-STR loci of 517 unrelated healthy individuals from Eastern China were genotypes by this kit. The distribution and frequency of haplotype were calculated and forensic parameters of the kit were assessed. Results The complete profiles can be obtained even when the PCR reaction volume with 6.25 μL. And correct profile was obtained with DNA down to 125 pg. No reproducible peaks were detected with the DNA of common animals and microorganism with the kit. For the male-male mixture testing, average 70% of the minor alleles were obtained when the ratios of 1∶19 and 19∶1. For the male-female mixture testing, results showed that the sensitivity of the kit was no compromised with the addition of female samples. Conclusion The validation studies demonstrated that Goldeneye®DNA ID 26Y system has good sensitivity and specificity, and suitable for mixture testing. The polymorphism of 26 Y-STR loci included in this kit are good for forensic application.

Key words: forensic genetics, Y-STR, Goldeneye?誖DNA ID 26Y system