Abstract: Objective To investigate the genetic polymorphism of 23 autosomal STR loci of HuaxiaTM Platinum kit in Chinese Han population, and to evaluate the forensic efficiency of HuaxiaTM Platinum kit. Methods A total of 500 unrelated healthy individuals from Han population were genotyped with HuaxiaTM Platinum kit. The frequency distribution and the parameter of population genetics of STR loci were analysed statistically. HuaxiaTM Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci, interior label, fluorescent mark, total number of alleles in Ladder and system effectiveness. Results All the 23 autosomal STR loci were consistent with Hardy-Weinberg equilibrium （P>0.05）. The discrimination power was 0.791 5-0.986 2. The polymorphism information content （PIC） was 0.559 0-0.914 0. The combined discrimination power （CDP） was 1-4.1×10-28, while combined probability of paternity exclusion in trio （CPET） and in duo （CPED） were 1-4.1×10-10 and 1-8.4×10-7, respectively. Compared with other 7 kits, HuaxiaTM Platinum kit contained the most number of alleles within the Ladder. Conclusion All the 23 autosomal STR loci of HuaxiaTM Platinum kit with highly polymorphic in Han population can be used for paternity testing and individual identification. Compared with other 7 kits, it appears that HuaxiaTM Platinum kit can provide more genetic information.

Key words: forensic genetics, short tandem repeat, polymorphism, genetic, HuaxiaTM Platinum kit, Han nationality