Abstract: In this paper we report a kind of double immunoenzyme labelled staining method (single enzyme method). The principle of the method is that HPR will whow blue -black color when it meet DABCoCL2/H2O2,while it will show brown color as it meet DAB/H2O2, two colors constract very remarkably. This method was used to detect the antigens of hearts and brains which obstained from forensic autopsy.The results showed that the method was sensitive. simple and econo-mical. One of the advantages is that the stained sections can keep for a long time. Thus,it is very paractical for forensic pathology.

Key words: Immunohistochemistry, Double staining, Forensic pathology