›› 2003, Vol. 0 ›› Issue (4): 199-200.

• 论文 • Previous Articles     Next Articles

DNA analysis of a 500 year mummy sample

TONG DA-YUE,WU XIN-YAO,CAI GUI-QING,ET AL.(FACULTY OF FORENSIC MEDICINE,ZHONGSHAN MEDICAL COL-LEGE,ZHONGSHAN UNIVERSITY,GUANGZHOU510089,CHINA)   

  • Online:2003-08-25 Published:2003-08-28

Abstract: Objective To accumulate experience for dated forensic matter anaylsis,for example,Mummy.Methods DNA are extracted by methods of phenol-chloroform and are purified by Wizard DNA clean-up system.The STRs locus are ampolification with Promega Powerplus16system.The mtDNA hypervariable region1(HV1)is amplificated by'3pair primers'.The products were sequenced with377DNA sequencer.Results The STRs locus very distinctness and mtDNA sequence is correct.Conclusion It is a valuable method for special forensic matters.

Key words: Mummy, DNA, mitochondrial, dated matter