Abstract: Objective To study the changes of liver phosphofructokinase-2(PFK-2) level at different postmortem intervals as well as due to different causes of death.Methods One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding,suffocating,and neck breaking,respectively.The content of liver PFK-2 at 0,1,2,4,8,12 and 24 hours following death were studied using immunohistochemishtry and image analysis.Results PFK-2 content of the rat's liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups.Conclusion PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.

Key words: postmortem interval, phosphofructokinase-2(PFK-2), content, liver tissue