Research Progress on Developmental Biology of Sarcosaprophagous Insects

doi:10.12116/j.issn.1004-5619.2020.401216

Abstract

Abstract:

Forensic entomology provides a feasible way to estimate postmortem interval （PMI）, of which the growth and development of sarcosaprophagous insects is the most widely used indicator in forensic practice. Over the years, forensic entomologists have carried out a large number of studies on the development biology of sarcosaprophagous insects. This paper illustrates the main factors that affect the development of sarcosaprophagous insects, including temperature, humidity, light, food types and poisons. The development indicators of sarcosaprophagous insects were reviewed from the perspectives of morphology, differential gene expression and biochemical characteristics. It is emphasized that future research of development biology on sarcosaprophagous insects should fully absorb and integrate the methods of artificial intelligence and omics, and the research object also needs further expansion in order to establish a more objective and more accurate PMI estimation method.

Key words: forensic pathology, forensic entomology, developmental biology, sarcosaprophagous insects, postmortem interval, review

CLC Number:

DF795.1

Yu WANG, Qiang GONG, Zhen-jiang LIU, Man WANG, Wang XU, Ying-hui WANG, Jiang-feng WANG. Research Progress on Developmental Biology of Sarcosaprophagous Insects[J]. Journal of Forensic Medicine, 2021, 37(5): 673-682.

References 83

1	BYRD J H， CASTNER J L. Forensic entomology：The utility of arthropods in legal investigations[M]. New York： CRC press，2009.
2	AMENDT J， CAMPOBASSO C P， GAUDRY E， et al. Best practice in forensic entomology-standards and guidelines[J]. Int J Legal Med，2007，121（2）：90-104. doi：10.1007/s00414-006-0086-x.
3	WANG Y， HU G， ZHANG Y， et al. Development of Muscina stabulans at constant temperatures with implications for minimum postmortem interval estimation[J]. Forensic Sci Int，2019，298：71-79. doi：10.1016/j.forsciint.2019.02.051.
4	VILLET M H， AMENDT J. Advances in entomological methods for death time estimation[M]//TURK E. Forensic pathology reviews. Berlin：Springer，2011：213-237.
5	吴晓晶，刘树生，郑中良. 变温对两种昆虫发育速率的影响[J]. 昆虫知识，1994，31（4）：237-240.
	WU X J， LIU S S， ZHENG Z L. The influence of variable temperature upon rate of development in two insects[J]. Entomol Knowl，1994，31（4）：237-240.
6	WARREN J A， ANDERSON G S. Effect of fluctuating temperatures on the development of a forensically important blow fly， Protophormia terraenovae （Diptera：Calliphoridae）[J]. Environ Entomol，2013，42（1）：167-172. doi：10.1603/EN12123.
7	NIEDEREGGER S， PASTUSCHEK J， MALL G. Preliminary studies of the influence of fluctuating temperatures on the development of various forensically relevant flies[J]. Forensic Sci Int，2010，199（1/2/3）：72-78. doi：10.1016/j.forsciint.2010.03.015.
8	SHI P， GE F. A comparison of different thermal performance functions describing temperature-dependent development rates[J]. J Therm Biol，2010，35（5）：225-231. doi：10.1016/j.jtherbio.2010.05.005.
9	ABALLAY F H， MURUA A F， ACOSTA J C， et al. Succession of carrion fauna in the arid region of San Juan Province， Argentina and its forensic relevance[J]. Neotropical Entomol，2012，41（1）：27-31. doi：10.1007/s13744-011-0005-9.
10	VALDES-PEREZGASGA M T， SANCHEZ-RAMOS F J， GARCIA-MARTINEZ O， et al. Arthropods of forensic importance on pig carrion in the Coahuilan Semidesert， Mexico[J]. J Forensic Sci，2010，55（4）：1098-1101. doi：10.1111/j.1556-4029.2010.01381.x.
11	FARIA L S， PASETO M L， FRANCO F T， et al. Insects breeding in pig carrion in two environments of a rural area of the State of Minas Gerais， Brazil[J]. Neotrop Entomol，2013，42（2）：216-222. doi：10.1007/s13744-012-0099-8.
12	WANG Y， LI L L， WANG J F， et al. Development of the green bottle fly Lucilia illustris at constant temperatures[J]. Forensic Sci Int，2016，267：136-144. doi：10.1016/j.forsciint.2016.07.019.
13	郭建波. 出口农副产品仓储昆虫检疫调查及白腹皮蠹生态学特性研究[D]. 扬州：扬州大学，2005.
	GUO J B. Quarantine investigation on stored grain insects in exportation agricultural products and study on ecological characteristics of Dermestes maculatus[D]. Yangzhou：Yangzhou University，2005.
14	BELLEMARE E R， BRUNELLE L. Larval and pupal development of Dermestes maculatus Degeer under controlled conditions of temperature and relative humidity[J]. Can Entomol，1950，82（1）：22-24. doi：10.4039/Ent8222-1.
15	GEORGE K A， ARCHER M S， TOOP T. Nocturnal colonization behavior of blowflies （Diptera：Calliphoridae） in Southeastern Australia[J]. J Forensic Sci，2013，58（S1）：S112-S116. doi：10.1111/j.1556-402 9.2012.02277.x.
16	BARNES K M， GRACE K A， BULLING M T. Nocturnal oviposition behavior of forensically important Diptera in Central England[J]. J Forensic Sci，2015，60（6）：1601-1604. doi：10.1111/1556-4029. 12841.
17	王江峰. 实用法医昆虫学[M].西安：西安交通大学出版社，2019.
	WANG J F. Practical forensic entomology[M]. Xi’an： Xi’an Jiaotong University Press，2019.
18	JOY J E， HERRELL M L， ROGERS P C. Larval fly activity on sunlit versus shaded raccoon carrion in southwestern West Virginia with special reference to the black blowfly （Diptera：Calliphoridae）[J]. J Med Entomol，2002，39（2）：392-397. doi：10.1603/0022-2585-39.2.392.
19	SHARANOWSKI B J， WALKER E G， ANDERSON G S. Insect succession and decomposition patterns on shaded and sunlit carrion in Saskatchewan in three different seasons[J]. Forensic Sci Int，2008，179（2/3）：219-240. doi：10.1016/j.forsciint.2008.05.019.
20	NABITY P D， HIGLEY L G， HENG-MOSS T M. Light-induced variability in development of forensically important blow fly Phormia regina （Diptera：Calliphoridae）[J]. J Med Entomol，2007，44（2）：351-358. doi：10.1093/jmedent/44.2.351.
21	BAUER A M， BAUER A， TOMBERLIN J K. Effects of photoperiod on the development of forensically important blow fly Chrysomya rufifacies （Diptera：Calliphoridae）[J]. J Med Entomol，2020，57（5）：1382-1389. doi：10.1093/jme/tjaa058.
22	王江峰，胡萃，闵建雄. 不同尸体材料上蝇类滋生及丝光绿绳Lucilia sericata生长发育比较[J]. 寄生虫与医学昆虫学报，1999，6（1）：52-57.
	WANG J F， HU C， MIN J X. Comparison of flies inhabiting in different carcass material and the development of Lucilia sericata （Diptera Calliphoridae） on above material[J]. Jishengchong Yu Yixue Kunchong Xuebao，1999，6（1）：52-57.
23	CLARK K， EVANS L， WALL R. Growth rates of the blowfly， Lucilia sericata， on different body tissues[J]. Forensic Sci Int，2006，156（2/3）：145-149. doi：10.1016/j.forsciint.2004.12.025.
24	HARNDEN L M， TOMBERLIN J K. Effects of temperature and diet on black soldier fly， Hermetia illucens （L.）（Diptera：Stratiomyidae），development[J]. Forensic Sci Int，2016，266：109-116. doi：10.1016/j.forsciint.2016.05.007.
25	FLORES M， LONGNECKER M， TOMBERLIN J K. Effects of temperature and tissue type on Chrysomya rufifacies （Diptera：Calliphoridae）（Macquart） development[J]. Forensic Sci Int，2014，245：24-29. doi：10.1016/j.forsciint.2014.09.023.
26	BERNHARDT V， SCHOMERUS C， VERHOFF M A， et al. Of pigs and men-Comparing the development of Calliphora vicina （Diptera：Calliphoridae） on human and porcine tissue[J]. Int J Legal Med，2017，131（3）：847-853. doi：10.1007/s00414-016-1487-0.
27	KANESHRAJAH G， TURNER B. Calliphora vicina larvae grow at different rates on different body tissues[J]. Int J Legal Med，2004，118（4）：242-244. doi：10.1007/s00414-004-0444-5.
28	DAY D M， WALLMAN J F. Influence of substrate tissue type on larval growth in Calliphora augur and Lucilia cuprina （Diptera：Calliphoridae）[J]. J Forensic Sci，2006，51（3）：657-663. doi：10.1111/j.1556-4029.2006.00127.x.
29	THYSSEN P J， DE SOUZA C M， SHIMAMOTO P M， et al. Rates of development of immatures of three species of Chrysomya （Diptera：Calliphoridae） reared in different types of animal tissues：Implications for estimating the postmortem interval[J]. Parasitol Res，2014，113（9）：3373-3380. doi：10.1007/s00436-014-4002-x.
30	王尧，万立华，李学博. 不同组织源食物对丝光绿蝇生长发育的影响[J].中国寄生虫学与寄生虫病杂志，2013，31（2）：124-126.
	WANG Y， WAN L H， LI X B. Effect of feeding on different tissues on larva development of Lucilia sericata[J]. Zhongguo Jishengchongxue Yu Jishengchongbing Zazhi，2013，31（2）：124-126.
31	EL-MOATY Z A， ABD ELMONEIM M K. Developmental variation of the blow fly Lucilia sericata （Meigen，1826）（Diptera：Calliphoridae） by different substrate tissue types[J]. J Asia-Pac Entomol，2013，16（3）：297-300. doi：10.1016/j.aspen.2013.03.008.
32	李学博，郑士立，王清山，等. 不同脂肪含量的肌肉食物对丝光绿蝇生长发育的影响[J].中国寄生虫学与寄生虫病杂志，2014，32（2）：135-138.
	LI X B， ZHENG S L， WANG Q S， et al. Effect of diets with different development fat levels on the body size and of Lucilia sericata[J]. Zhongguo Jishengchongxue Yu Jishengchongbing Zazhi，2014，32（2）：135-138.
33	O’BRIEN C， TURNER B. Impact of paracetamol on Calliphora vicina larval development[J]. Int J Legal Med，2004，118（4）：188-189. doi：10.1007/s00414-004-0440-9.
34	吕宙，翟仙敦，周海梅，等. 氯胺酮对大头金蝇生长发育的影响[J].中国寄生虫学与寄生虫病杂志，2012，30（5）：361-366.
	LÜ Z， ZHAI X D， ZHOU H M， et al. Effect of Ketamine on the development of Chrysomya megacephala （Diptera：Calliphoridae）[J]. Zhongguo Jishengchongxue Yu Jishengchongbing Zazhi，2012，30（5）：361-366.
35	ZOU Y， HUANG M， HUANG R， et al. Effect of ketamine on the development of Lucilia sericata （Meigen）（Diptera：Calliphoridae） and preliminary pathological observation of larvae[J]. Forensic Sci Int，2013，226（1/2/3）：273-281. doi：10.1016/j.forsciint.2013.01.042.
36	CARVALHO L M， LINHARES A X， TRIGO J R. Determination of drug levels and the effect of diazepam on the growth of necrophagous flies of forensic importance in southeastern Brazil[J]. Forensic Sci Int，2001，120（1/2）：140-144. doi：10.1016/s0379-0738（01）00421-2.
37	戴军. 安定对丝光绿蝇幼虫生长发育的影响及其法医学意义[D].石家庄：河北医科大学，2005.
	DAI J. Effect of diazepam on the growth and development of Lucilia sericata （Diptera Calliphoridae） and its forensic importance[D]. Shijiazhuang：Hebei Medical University，2005.
38	王丽娟. 不同温度下安定对大头金蝇生长发育的影响及其在死后间隔时间推断方面的应用[D]. 石家庄：河北医科大学，2006.
	WANG L J. Effect of diazepam in tissues on development of Chrysomya megacephala （Diptera：Calliphoridae） in different temperature and implication of this effect on estimation of postmortem intervals using arthropod development patterns[D]. Shijiazhuang： Hebei Medical University，2006.
39	DE CARVALHO L M L， LINHARES A X， PALHARES F A B. The effect of cocaine on the development rate of immatures and adults of Chrysomya albiceps and Chrysomya putoria （Diptera：Calliphoridae） and its importance to postmortem interval estimate[J]. Forensic Sci Int，2012，220（1/2/3）：27-32. doi：10.1016/j.forsciint.2012.01.023.
40	GOFF M L， OMORI A I， GOODBROD J R. Effect of cocaine in tissues on the development rate of Boettcherisca peregrina （Diptera：Sarcophagidae）[J]. J Med Entomol，1989，26（2）：91-93. doi：10.1093/jmedent/26.2.91.
41	KHARBOUCHE H， AUGSBURGER M， CHERIX D， et al. Codeine accumulation and elimination in larvae， pupae， and imago of the blowfly Lucilia sericata and effects on its development[J]. Int J Legal Med，2008，122（3）：205-211. doi：10.1007/s00414-007-0217-z.
42	RASHID R A， OSMAN K， ISMAIL M I， et al. Determination of malathion levels and the effect of malathion on the growth of Chrysomya megacephala （Fabricius） in malathion-exposed rat carcass[J]. Tropical Biomed，2008，25（3）：184-190.
43	TABOR K L， FELL R D， BREWSTER C C， et al. Effects of antemortem ingestion of ethanol on insect successional patterns and development of Phormia regina （Diptera：Calliphoridae）[J]. J Med Entomol，2005，42（3）：481-489. doi：10.1093/jmedent/42.3.481.
44	GOFF M L， BROWN W A， OMORI A I， et al. Preliminary observations of the effects of amitriptyline in decomposing tissues on the development of Parasarcophaga ruficornis （Diptera：Sarcophagidae） and implications of this effect to estimation of postmortem intervals[J]. J Forensic Sci，1993，38（2）：316-322.
45	PIEN K， LALOUP M， PIPELEERS-MARICHAL M， et al. Toxicological data and growth characteristics of single post-feeding larvae and puparia of Calliphora vicina （Diptera：Calliphoridae） obtained from a controlled nordiazepam study[J]. Int J Legal Med，2004，118（4）：190-193. doi：10.1007/s00414-004-0441-8.
46	SOUZA C M， THYSSEN P J， LINHARES A X. Effect of nandrolone decanoate on the development of three species of Chrysomya （Diptera：Calliphoridae），flies of forensic importance in Brazil[J]. J Med Entomol，2014，48（1）：111-117. doi：10.1603/me09291.
47	FERRAZ A C， DALLAVECCHIA D L， SILVA D C， et al. Effects of the antibiotics gentamicin on the postembryonic development of Chrysomya putoria （Diptera：Calliphoridae）[J]. J Insect Sci，2014，14（1）：1-5. doi：10.1093/jisesa/ieu141.
48	GOFF M L， BROWN W A， HEWADIKARAM K A， et al. Effect of heroin in decomposing tissues on the development rate of Boettcherisca peregrina （Diptera， Sarcophagidae） and implications of this effect on estimation of postmortem intervals using arthropod development patterns[J]. J Forensic Sci，1991，36（2）：537-542.
49	BOUREL B， HÉDOUIN V， MARTIN-BOUYER L， et al. Effects of morphine in decomposing bodies on the development of Lucilia sericata （Diptera：Calliphoridae）[J]. J Forensic Sci，1999，44（2）：354-358.
50	田洁. 吗啡对大头金蝇生长发育的影响及其在死后间隔时间推断方面的应用[D].石家庄：河北医科大学，2004.
	TIAN J. Effect of Morphine in tissues on development of Chrysomya megacephala （Diptera） and implication of this effect on estimation of postmortem intervals using arthropod development patterns[D]. Shijiazhuang： Hebei Medical University，2004.
51	赵文爱，王伯霞，胡圣爱，等. 恒温条件下吗啡对丝光绿蝇发育规律改变与死者死亡时间推断的相关研究[J]. 中国病原生物学杂志，2008，3（8）：612-615. doi：10.3969/j.issn.1673-5234.2008.08.018.
	ZHAO W A， WANG B X， HU S A， et al. The relationship between the postmortem interval and effects of morphine on the growth stage of Lucilia sericata[J]. Zhongguo Bingyuan Shengwuxue Zazhi，2008，3（8）：612-615.
52	GEORGE K A， ARCHER M S， GREEN L M， et al. Effect of morphine on the growth rate of Calliphora stygia （Fabricius）（Diptera：Calliphoridae） and possible implications for forensic entomology[J]. Forensic Sci Int，2009，193（1/2/3）：21-25. doi：10.1016/j.forsciint.2009.08.013.
53	MULLANY C， KELLER P A， NUGRAHA A S， et al. Effects of methamphetamine and its primary human metabolite， p-hydroxymethamphetamine， on the development of the Australian blowfly Calliphora stygia[J]. Forensic Sci Int，2014，241：102-111. doi：10.1016/j.forsciint.2014.05.003.
54	GOFF M L， BROWN W A， OMORI A I. Preliminary observations of the effect of methamphetamine in decomposing tissues on the development rate of Parasarcophaga ruficornis （Diptera：Sarcophagidae） and implications of this effect on the estimations of postmortem intervals[J]. J Forensic Sci，1992，37（3）：867-872.
55	王禹，王江峰. 法医昆虫学标准化应用及我国主要嗜尸性昆虫应用数据[J]. 法医学杂志，2018，34（5）：468-474. doi：10.12116/j.issn.1004-5619.2018.05.003.
	WANG Y， WANG J F. Standardized application of forensic entomology and application data of sarcos-aprophagous insects in China[J]. Fayixue Zazhi，2018，34（5）：468-474.
56	AMENDT J， RICHARDS C S， CAMPOBASSO C P， et al. Forensic entomology：Applications and limitations[J]. Forensic Sci Med Pathol，2011，7（4）：379-392. doi：10.1007/s12024-010-9209-2.
57	GRASSBERGER M， REITER C. Effect of temperature on Lucilia sericata （Diptera：Calliphoridae） development with special reference to the isomegalen- and isomorphen-diagram[J]. Forensic Sci Int，2001，120（1/2）：32-36. doi：10.1016/S0379-0738（01）00413-3.
58	WANG Y， ZHANG Y， HU G， et al. Development of Megaselia spiracularis （Diptera：Phoridae） at different constant temperatures[J]. J Therm Biol，2020，93：102722. doi：10.1016/j.jtherbio.2020.102722.
59	IKEMOTO T， TAKAI K. A new linearized formula for the law of total effective temperature and the evaluation of line-fitting methods with both variables subject to error[J]. Environ Entomol，2000，29（4）：671-682. doi：10.1603/0046-225X-29.4.671.
60	VOSS S C， SPAFFORD H， DADOUR I R. Temperature-dependant development of Nasonia vitripennis on five forensically important carrion fly species[J]. Entomol Exp Appl，2010，135（1）：37-47. doi：10.1111/j.1570-7458.2010.00966.x.
61	BROWN K， THORNE A， HARVEY M. Calliphora vicina （Diptera：Calliphoridae） pupae：A timeline of external morphological development and a new age and PMI estimation tool[J]. Int J Legal Med，2015，129（4）：835-850. doi：10.1007/s00414-014-1068-z.
62	BARROS-CORDEIRO K B， BÁO S N， PUJOL-LUZ J R. Intra-puparial development of the black soldier-fly， Hermetia illucens[J]. J Insect Sci，2014，14（1）：83. doi：10.1093/jis/14.1.83.
63	PUJOL-LUZ J R， BARROS-CORDEIRO K B. Intra-puparial development of the females of Chrysomya albiceps （Wiedemann）（Diptera， Calliphoridae）[J]. Rev Bras Entomol，2012，56（3）：269-272.
64	BROWN K， HARVEY M. Optical coherence tomography：Age estimation of Calliphora vicina pupae in vivo?[J]. Forensic Sci Int，2014，242：157-161. doi：10.1016/j.forsciint.2014.07.001.
65	VOSS S C， MAGNI P， DADOUR I， et al. Reflectance-based determination of age and species of blowfly puparia[J]. Int J Legal Med，2017，131（1）：263-274. doi：10.1007/s00414-016-1458-5.
66	MARTÍN-VEGA D， SIMONSEN T J， WICKLEIN M， et al. Age estimation during the blow fly intra-puparial period：A qualitative and quantitative approach using micro-computed tomography[J]. Int J Legal Med，2017，131（5）：1429-1448. doi：10.1007/s00414-017-1598-2.
67	ARBEITMAN M N， FURLONG E E M， IMAM F， et al. Gene expression during the life cycle of Drosophila melanogaster[J]. Science，2002，297（5590）：2270-2275. doi：10.1126/science.1072152.
68	TARONE A M， JENNINGS K C， FORAN D R. Aging blow fly eggs using gene expression：A feasibility study[J]. J Forensic Sci，2007，52（6）：1350-1354. doi：10.1111/j.1556-4029.2007.00587.x.
69	TARONE A M， FORAN D R. Gene expression during blow fly development：Improving the precision of age estimates in forensic entomology[J]. J Forensic Sci，2011，56：S112-S122. doi：10.1111/j.1556- 4029.2010.01632.x.
70	BOEHME P， SPAHN P， AMENDT J， et al. Differential gene expression during metamorphosis：A promising approach for age estimation of forensically important Calliphora vicina pupae （Diptera：Calliphoridae）[J]. Int J Leg Med，2013，127（1）：243-249. doi：10.1007/s00414-012-0699-1.
71	BOEHME P， SPAHN P， AMENDT J， et al. The analysis of temporal gene expression to estimate the age of forensically important blow fly pupae：Results from three blind studies[J]. Int J Leg Med，2014，128（3）：565-573. doi：10.1007/s00414-013-0922-8.
72	WANG Y， GU Z， XIA S， et al. Estimating the age of Lucilia illustris during the intrapuparial period using two approaches：Morphological changes and differential gene expression [J]. Forensic Sci Int，2018，287：1-11. doi：10.1016/j.forsciint.2018.02.025.
73	WANG Y， GU Z， LI L， et al. Gene expression during the intra-puparial stage of Chrysomya megacephala：Implications for postmortem interval estimation[J]. J Asia-Pac Entomol，2019，22（3）：841-846. doi：10.1016/j.aspen.2019.06.012.
74	LIU Z Y， HAN H， CHEN W， et al. Evaluation of reference genes and age estimation of forensically useful Aldrichina grahami （Diptera：Calliphoridae） during intrapuparial period[J]. J Med Entomol，2021，58（1）：47-55. doi：10.1093/jme/tjaa144.
75	SHANG Y J， REN L P， YANG L， et al. Differential gene expression for age estimation of forensically important Sarcophaga peregrina （Diptera：Sarcophagidae） intrapuparial[J]. J Med Entomol，2020，57（1）：65-77. doi：10.1093/jme/tjz137.
76	朱光辉，叶恭银，胡萃. 巨尾阿丽蝇幼期血淋巴可溶性蛋白组成的时间特征[J]. 浙江大学学报（农业与生命科学版），2004，30（1）：69-72. doi：10.3321/j.issn：1008-9209.2004.01.015.
	ZHU G H， YE G Y， HU C. Development profile of hemolymph soluble proteins during the larval and pupal stages in Aldrichina grahami[J]. Zhejiang Daxue Xuebao （Agric Life Sci），2004，30（1）：69-72.
77	李凯. 尸食性蝇类的分子鉴别及其发育生物化学特征用于死后间隔时间推断的基础研究[D]. 杭州：浙江大学，2006.
	LI K. Fundamental research on the application of molecular identification and biochemical characters of necrophagous flies in the determination of postmortem interval[D].Hangzhou：Zhejiang University，2006.
78	EL-SHESHENY I， EL HAWARY I， MESBAH I， et al. Comparative proteomic analysis between fifth-instar nymphs and adults of Asian citrus psyllid Diaphorina citri[J]. Physiol Entomol，2016，41（2）：162-184. doi：10.1111/phen.12139.
79	ZHENG A J， LI J K， BEGNA D， et al. Proteomic analysis of honeybee （Apis mellifera L.） pupae head development[J]. PLoS One，2011，6（5）：e20428. doi：10.1371/journal.pone.0020428.
80	LI Q， WANG D， LÜ S， et al. Comparative proteomics and expression analysis of five genes in Epicauta chinensis larvae from the first to fifth instar[J]. PLoS One，2014，9（2）：e89607. doi：10.1371/journal.pone.0089607.
81	LI Y， WANG X， HOU Y， et al. Integrative proteomics and metabolomics analysis of insect larva brain：Novel insights into the molecular mechanism of insect wandering behavior[J]. J Proteome Res，2016，15（1）：193-204. doi：10.1021/acs.jproteome.5b00736.
82	BUTCHER J B， MOORE H E， DAY C R， et al. Artificial neural network analysis of hydrocarbon profiles for the ageing of Lucilia sericata for post mortem interval estimation[J]. Forensic Sci Int，2013，232（1/2/3）：25-31. doi：10.1016/j.forsciint.2013.0 6.018.
83	MOORE H E， BUTCHER J B， DAY C R， et al. Adult fly age estimations using cuticular hydrocarbons and Artificial Neural Networks in forensically important Calliphoridae species[J]. Forensic Sci Int，2017，280：233-244. doi：10.1016/j.forsciint.2017.10.001.

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