Abstract: Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping. Methods Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by para- magnetic particle method. DNA was quantified by PCR with QuantifilerTM Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpF■STR IdentifilerTM PCR Amplification Kit using 3130XL-Avant genetic analyzer. Results The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218±0.041) ng/μL. Conclusion Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.

Key words: forensic genetics, entomology, extraction method, phenol-chloroform method, paramagnetic particle method, muscidae