Abstract: Objective To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS). Methods Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O -bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC -MS/MS. Results The detection limit of EtG in blood was 0.05 μg/mL. Calibration curve covered a span from 0.1-10 μg/mL with a good linear relationship (r=0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG. Conclusion The method is suitable for blood EtG analysis.

Key words: forensic toxicology, blood, ethyl glucuronide, GC-MS/MS