Abstract: Objective The methods for identification of sibling sisters were explored with detection of genetic markers on autochromosome and X-chromosome.Methods Genomic DNA of the sibling sisters were extracted,and 15 STRs on autochromosome and 17 STRs on X-chromosome were genotyped by SinofilerTM kit,Mentype Argus X-8 kit and in-house kit of X-STRs,respectively.11 X-SNPs were genotyped with TaqMan technology.Results Full sibling relationship of the test samples were confirmed by calculating full sibling index of STRs in autochromosome,which were also supported by the detection of 1-2 same alleles at each locus on X-chromosome.Conclusion In identification of full sibling sister,not only STRs on autochromosome but also polymorphism genetic markers in X-chromosome can be utilized.

Key words: forensic genetics, sibling relations, X chromosome, short tandem repeat, single nucleotide polymorphism