Abstract: Objective To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR（DOP-PCR） and to explore its reliability and sensitivity. Methods DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system. Results DOP-PCR system was successfully established and the detection sensitivity reached 5 cells （30 pg） by pretreatment of DOP-PCR and then detection of STR genotyping. Conclusion The system established in this study is reliable and more testing sensitive for forensic trace evidence.

Key words: forensic genetics, gene amplification, degenerate oligonucleotide primed-PCR