Abstract: Objective To explore the forensic application value of detection of matrix metalloproteinase-11（MMP-11） in menstrual blood by enhanced chemiluminescence method. Methods Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated. Results The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 μL sample was added, the mass concentration of protein was 1.329 μg/μL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 ℃ for 20 months. Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.

Key words: forensic genetics, luminescent measurements, matrix metalloproteinase-11, menstrual blood