Abstract: Objective To determine the normal range of the 33 elements （Li, Be, B, Mg, Al, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Zr, Mo, Ag, Cd, Sb, Cs, Ba, Au, Hg, Tl, Pb, Th and U） in human whole blood of general population in Hunan province. Methods Blood samples were analyzed by inductively coupled plasma-mass spectrometry （ICP-MS） to determine the normal range. The influences of district, gender and age to the element content in blood samples were also observed. Results The normal range of 33 elements in blood samples from general population in Hunan province were obtained. Gender was shown to statistically influence the concentrations of B, Mg, Ca, Ti, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Cs, Hg and Pb （P<0.05）, while age was shown to influence the concentrations of Co, Ni, Cs and Hg in women （P<0.05） as well as Cu, Se and Hg in men（P<0.05）. Conclusion Although there are variables in different districts, the normal ranges of trace element in blood of the four cities in Hunan province are established.

Key words: forensic toxicology, elements, blood, inductively coupled plasma-mass spectrometry, Hunan