›› 2017, Vol. 33 ›› Issue (5): 514-515.转521.DOI: 10.3969/j.issn.1004-5619.2017.05.014

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PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

DING SHAO-CHENG, ZHANG HUAI-CAI, GAO LIN-LIN   

  1. Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310016, China
  • Online:2017-10-25 Published:2017-10-28

Abstract: Objective To establish a convenient and rapid method for extracting DNA from bone. Methods Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTATM lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis. Results STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%. Conclusion The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying.

Key words: forensic genetics, bone, DNA, PrepFiler Express BTATM, silica-extraction method

CLC Number: