Abstract: Objective To investigate the genetic data of 21 autosomal STR included in GoldeneyeTM DNA ID 22NC Kit in Chinese Han nationality and to evaluate the forensic application. Methods By detected 500 unrelated healthy individuals in Chinese Han nationality of East China with GoldeneyeTM DNA ID 22NC Kit, allele frequencies, population genetics parameters and linkage disequilibrium information of the 21 autosomal STR were statistically analyzed. Results In the 21 autosomal STR, no deviations from Hardy-Weinberg equilibrium were detected and all loci were independent form each other. DP values of 21 autosomal STR were all above 0.85, and the combined discrimination power was 1-3.616　5×10-26. Combined mean exclusion chance of this system in duo cases was 1-2.786　81×10-6, in trio cases was 1-8.545　82×10-10. Conclusion Twenty-one autosomal STR included in GoldeneyeTM DNA ID 22NC Kit are highly polymorphic in the Han nationality. Combined with GoldeneyeTM DNA ID 20A Kit, the kit can satisfy the needs for full-sibling testing and facilitate the solution of this kind of case tools.

Key words: forensic genetics, polymorphism, genetics, short tandem repeat, GoldeneyeTM DNA ID 22NC Kit