Abstract: Objective To test and estimate the forensic application of GoldeneyeTM DNA ID 25A Kit. Methods The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. Results The peak height balance of the different loci was ≥42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. Conclusion GoldeneyeTM DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.

Key words: forensic genetics, GoldeneyeTM DNA ID 25A Kit, validation test