›› 2015, Vol. 31 ›› Issue (6): 454-455.DOI: 10.3969/j.issn.1004-5619.2015.06.010

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DNA Extraction of Cast-off Cells of Fingerprints from 502 Glue Fumigated Contact Samples

WANG XIAN-WEN1, LENG XUE-FENG1, WANG SHOU-YU2   

  1. 1. Criminal Police Branch, Haikou Public Security Bureau, Haikou 570208, China; 2. Criminal Police Brigade of Qiongshan Branch, Haikou Public Security Bureau, Haikou 571100, China
  • Online:2015-12-25 Published:2015-12-28

Abstract: Objective To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples. Methods Sixty-six cases were visualized by 502 glue fingerprint fumigation. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints from testing samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification. Results In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1 000-4 000 RFU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method. Conclusion The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic science practice.

Key words: forensic genetics, DNA, cells, acetone, fingerprint, adhesives

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