Journal of Forensic Medicine

Journal of Forensic Medicine ›› 2020, Vol. 36 ›› Issue (5): 682-687.DOI: 10.12116/j.issn.1004-5619.2020.05.014

• Original Articles • Previous Articles     Next Articles

Influence of Halogenated Hydroxyl-Alkanes Inhalation Anesthetic on the Determination of Ethanol Content in Blood

XIE Bing1 , YAN Wen- jing2 , MENG Xian- yue3 , MIAO Xin- gang1 , YU Feng1 , DONG Mei1 , SHI Yan4 , XIANG Ping4 , MA Chun-ling1 , WEN Di1   

  1. 1. Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 2. Huanghua Forensic Identification Center, Huanghua 061100, Hebei Province, China; 3. Shijiazhuang Public Transportation Administration Bureau, Shijiazhuang 050091, China; 4. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
  • Received:2019-12-04 Online:2020-10-25 Published:2020-10-28

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Abstract: Objective To study the influence of halogenated hydroxyl-alkanes inhalation anesthetic on the determination of ethanol content in blood. Methods Halogenated hydroxyl-alkanes were analyzed by headspace gas chromatography with double column confirmatory detection method. The influence of halogenated hydroxyl-alkanes on determination of ethanol content in blood sample by headspace gas chromatography was explored under the different detection conditions of KB-BAC1/ KB-BAC2 and J&W DB-ALC1/DB-ALC2 gas chromatographic column. Results The retention time of sevoflurane and enflurane was similar to that of ethanol and tert butanol respectively when using the J&W DB-ALC1/DB-ALC2 gas chromatographic column, and interfered with the detection of ethanol content in blood; only J&W DB-ALC1 gas chromatographic column can separate the sevoflurane and ethanol components, so as to eliminate their influence on the detection of ethanol content in blood. When using KB- BAC1/KB-BAC2 gas chromatographic column, the retention time of sevoflurane, isoflurane and ethanol is similar, especially that of sevoflurane and ethanol, and sevoflurane obviously interferes with the determination of ethanol content in blood. Conclusion Halogenated hydroxy-alkanes interfere with determination of ethanol content in blood by headspace gas chromatography. The interference can be discriminated effectively by choosing the suitable chromatographic column and double column confirmatory detection

Key words: forensic toxicology, halogenated hydroxy-alkanes, anesthetics, inhalation, ethanol, headspace gas chromatography, blood

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