法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (1): 5-10.DOI: 10.12116/j.issn.1004-5619.2019.01.002

• 论著 • 上一篇    下一篇

小鼠皮肤切创愈合过程中膜联蛋白A1的表达

金馨1，2，3， 赵建新1，2，3， 姚艺1，2，3， 黄俊杰1，2，3， 范琰琰1，2，3， 喻林升1，2，3   

  1. 1. 温州医科大学法医学系，浙江 温州325035； 2. 温州医科大学司法鉴定中心，浙江 温州325035； 3. 温州医科大学司法鉴定科学技术研究所，浙江 温州325035
  • 发布日期:2019-02-25 出版日期:2019-02-28
  • 通讯作者: 喻林升，男，博士，教授，主要从事法医病理学研究；E-mail：yls@wmu.edu.cn
  • 作者简介:金馨（1993—），女，硕士研究生，主要从事法医病理学研究；E-mail：jinxin_2016@126.com

Expression of Annexin A1 during Skin Incised Wound Healing in Mice

JIN Xin1,2,3, ZHAO Jian-xin1,2,3, YAO Yi1,2,3, HUANG Jun-jie1,2,3, FAN Yan-yan1,2,3, YU Lin-sheng1,2,3   

  1. 1. Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China; 2. Judicial Forensic Center, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China; 3. Institute of Forensic Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China
  • Online:2019-02-25 Published:2019-02-28

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摘要： 目的 通过检测皮肤切创愈合过程中膜联蛋白A1（annexin A1，ANXA1）的表达变化，探讨其在皮肤损伤修复过程中的表达变化规律及作用。 方法 制作小鼠皮肤损伤模型，分别取对照组和损伤后6 h、12 h、1 d、3 d、5 d、7 d、10 d、14 d的损伤处皮肤组织，应用苏木素-伊红（hematoxylin-eosin，HE）染色观察创口形态学变化，应用免疫组织化学技术及蛋白质印迹法检测ANXA1的表达。 结果 HE染色显示皮肤创口正常愈合。免疫组织化学结果显示：对照组ANXA1表达于表皮、毛囊、皮脂腺、血管内皮；实验组伤后6~12 h在创口周边区表皮及皮肤附件中ANXA1表达增强，在中性粒细胞和少量单核细胞中呈高表达；伤后1~3 d ANXA1阳性表达以单核细胞为主；伤后5~10 d，ANXA1阳性表达以新生血管内皮及成纤维细胞为主；伤后14 d仍有少量成纤维细胞表达ANXA1。蛋白质印迹法检测结果显示，伤后6 h与对照组相比，ANXA1表达明显升高，在1 d时达到峰值，之后逐渐下降。 结论 ANXA1可能参与了皮肤损伤修复的调控，在愈合过程中呈一定的时序性变化，有望成为推断创口形成时间的生物学指标。

关键词: 法医病理学, 创伤和损伤, 皮肤, 膜联蛋白A1, 损伤时间推断, 小鼠

Abstract: Objective To investigate the expression changes of annexin A1 （ANXA1） during the process of skin incision healing, and to explore its expression and function during skin injury repair. Methods The skin injury model of mice was prepared, and skin tissues of the controls and the injured group at 6 h, 12 h, 1 d, 3 d, 5 d, 7 d, 10 d and 14 d after injuries were taken. The morphological changes of the wound were observed by hematoxylin-eosin （HE） staining, and the expression of ANXA1 was detected by immunohistochemistry （IHC） and Western blotting. Results HE staining showed normal healing of skin wounds. IHC results revealed that ANXA1 was expressed in the epidermis, hair follicle, sebaceous gland and vascular endothelium. In the injured group, the expression of ANXA1 was enhanced in epidermis and skin appendages around the wound 6-12 h after injury, and ANXA1 was also highly expressed in neutrophils and a small number of mononuclear cells. ANXA1 was mainly positively expressed in monocytes, neovascular endothelial cells and fibroblasts, and small amount of fibroblasts at 1-3 d, 5-10 d, and 14 d after injury, respectively. Western blotting showed that, compared with the controls, the expression of ANXA1 was significantly increased at 6 h after injury, peaked at 1 d, and then decreased gradually in the injured group. Conclusion ANXA1 may be involved in the regulation of skin damage repair, with time-dependent expression during skin wound healing, and thus is expected to be a biological marker for inferring the wound formation time.

Key words: forensic pathology, trauma and injury, skin, annexin A1, wound age determination, mice