法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (1): 52-54.DOI: 10.3969/j.issn.1004-5619.2012.01.013

• 综述 • 上一篇    下一篇

疑难生物检材法医DNA检验的现状与进展

党华伟1，毛  炯1，王  惠1，黄江平2，白小刚1   

  1. （1. 成都市公安局物证鉴定所，四川 成都 610081； 2. 上海市公安局物证鉴定中心，上海 200083）
  • 发布日期:2012-02-25 出版日期:2012-02-28
  • 作者简介:党华伟（1983—），男，浙江杭州人，主要从事微量生物检材DNA检验方面的研究；E-mail：danghuawei@yahoo.cn

Research Status and Prospects of DNA Test on Difficult Specimens

DANG HUA-WEI1, MAO JIONG1, WANG HUI1, HUANG JIANG-PING2, BAI XIAO-GANG1   

  1. （1. Institute of Forensic Science, Chengdu Public Security Bureau, Chengdu 610081, China; 2. Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China）
  • Online:2012-02-25 Published:2012-02-28

摘要： 本文对法医DNA检验中遇到的降解生物检材、微量检材、混合生物检材三大类疑难检材的检验现状和研究进展进行了综述，分析了各种疑难检材的来源、处理方法和注意事项等，介绍了近年来在疑难检材的法医DNA检验中发展起来的一些新方法如利用线粒体检验系统、改变原有实验条件、低体积扩增等和一些新技术如全基因组扩增技术、激光捕获显微切割技术和mini-STR技术等。

关键词: 法医遗传学, 检材, 综述[文献类型]

Abstract: This paper reviews the advances of DNA detection on three types of difficult biological specimens including degraded samples, trace evidences and mixed samples. The source of different samples, processing methods and announcements were analyzed. New methods such as mitochondrial test system, changing the original experimental conditions, low-volume PCR amplification and new technologies such as whole genome amplification techniques, laser capture micro-dissection, and mini-STR technology in recent years are introduced.

Key words: forensic genetics, samples, review[publication type]

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