法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (5): 342-346.DOI: 10.3969/j.issn.1004-5619.2012.05.005

• 论著 • 上一篇    下一篇

冷冻研磨联合超声波技术处理指甲检材

陈  航1，2，向  平2，孙其然2，沈  敏2   

  1. （1. 复旦大学上海医学院法医学系，上海 200032； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2012-10-25 出版日期:2012-10-28
  • 通讯作者: 沈敏，女，研究员，博士研究生导师，主要从事法医毒物学研究；E-mail：minshensfjd@hotmail.com
  • 作者简介:陈航（1988—），男，上海人，博士研究生，主要从事法医毒物学研究；E-mail：Dio.Chen.Hang@gmail.com
  • 基金资助:

    国家自然科学基金资助项目（20975070）；上海市法医学重点实验室基金资助项目（12DZ2271500）

Freeze Grinding Combined Ultrasonic Technique for the Treatment of Nail Test Material

CHEN HANG1,2, XIANG PING2, SUN QI-RAN2, SHEN MIN2   

  1. （1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2012-10-25 Published:2012-10-28

被引次数

1

摘要： 目的 探讨冷冻研磨联合超声波技术在毒（药）物分析中，作为指甲样品前处理方法的可能性，并比较该技术与其他传统前处理方法的优劣。 方法 考察5种指甲前处理方法，结合扫描电子显微镜方法从微观结构观察指甲变化。 结果 碱处理后指甲显微结构被完全破坏，酸水解后指甲内部层级式结构更为明显且略紧致，甲醇浸泡后层级结构模糊。超声波处理的剪碎指甲呈被击碎状展开，冷冻研磨联合超声波处理后指甲粉末保持原形，且粒径较剪碎指甲小100倍。 结论 冷冻研磨联合超声波技术能提高样品前处理过程中毒（药）物的释放效率，并保证毒（药）物稳定性，该法适用于指甲中的毒（药）物分析。

关键词: 法医毒理学, 指（趾）甲, 显微镜检查, 电子, 扫描, 超声波, 冷冻研磨

Abstract: Objective To investigate the feasibility of the new method of combining freeze grinding with ultrasonic technique for the pretreatment of the nail for toxicological and pharmaceutical analysis and to compare the advantages and disadvantages of this method with other traditional methods. Methods Five pretreatment methods were examined. Scanning electron microscope （SEM） was used to observe the micro-structural changes of the nail. Results The microscopic structure of nail totally destroyed after alkali treatment. The hierarchy mode of the internal structure became obvious and tight after acid hydrolysis, which became indistinct after methanol infiltration. The structure of nail broke to pieces after ultrasonic technique. After freeze grinding combined ultrasonic technique, the particle structure kept original shape, and its size was one hundred times smaller than which after manual way. Conclusion The freeze grinding combined ultrasonic technique can improve the release efficiency, and ensure the stability of the toxicant or drug during the pretreatment process. It is appropriate for toxicological and pharmaceutical analysis in the nail.

Key words: forensic toxicology, nails, microscopy, electron, scanning, ultrasonic wave, freeze grinding

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