法医学杂志

法医学杂志 ›› 1998, Vol. 14 ›› Issue (4): 204-206+.

• 论文 • 上一篇    下一篇

用PCR-SSP法分析中国辽宁汉族HLA-DRBI基因多态性

刘利民,胡姝,贾静涛   

  1. 中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001
  • 发布日期:1998-08-25 出版日期:1998-08-28

The polymophic study of HLA-DRB1 gene in Chinese Liaoning Han with PCR-Sequence specific primers

LIU LIMIN, HU SHU, JIA JINGTAO (DEPARTMENT OF FORENSICMEDICINE, CHINA MEDIAL UNIVERSISY, SHENYANG 110001)   

  • Online:1998-08-25 Published:1998-08-28

被引次数

13

摘要： 应用PCR－SSP方法对辽宁地区159名无关个体进行HLA－DRB1位点基因分型，检出8组等位基因（扩增片段大小为100bp），基因频率范围在0．02201～0．23899。36种可能基因型中检出33种。经x2检验符合Hardy－Weinberg平衡定律。本地区汉族群体的期望杂合度为85％，观察杂合度为83％。个人鉴别机率（DP）为0．94非父排除率（EPP）为66％。本法具有简单、快速、结果可靠的特点，不仅适用于法医学亲子鉴定和个人识别、移植配型，亦可用于相关疾病及人类遗传学研究。

关键词: HLA－DRBI, 基因, PCR, 基因频率

Abstract: 8 allele and group specificity at HLA - DRB1 locuswere genotyped in 159 Unrelated individuals of Liaoning Han,with polymerase chain reaction - sequence specific primers.The range of gene frequencies was from 0 .02201 - 0 .23899.Heterozagosity was 83%. 33 out of 36 theoretical genotypeswere identified and the result conformed to Hardy- Weinbergequilibrium law. The present method can be used not only inpaternity test, individual identification, but also in clinicgraft matching between donor and recipient as well as thestudy of HLA.

Key words: HLA-DRB1 gene, PCR, gene frequency