法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (2): 107-109+.

• 论文 • 上一篇    下一篇

毒鼠强中毒实质性器官组织超微病理变化研究

朱传红,刘良,刘艳,黄光照   

  1. 华中科技大学同济医学院法医学系法医病理学教研室,华中科技大学同济医学院法医学系法医病理学教研室,华中科技大学同济医学院法医学系法医病理学教研室,华中科技大学同济医学院法医学系法医病理学教研室 湖北武汉430030武汉市公安局刑事侦查局湖北武汉430019 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030
  • 发布日期:2005-04-25 出版日期:2005-04-28

Study on Ultra-structural Pathological Changes of Rats Poisoned by Tetramine

ZHU CHUAN-HONG1,2, LIU LIANG1, LIU YAN1, ET AL. (1. DEPARTMENT OF MEDICOLEGAL, TONGJI MEDICAL COLLEGE, HUAZHONG SCIENCE AND TECHNOLOGY UNIVERSITY, WUHAN 430030,CHINA; 2.DEPARTMENT OF CRIMINAL POLICE, WUHAN PUBLIC SECURITY BUREAU, WUHAN 430019, CHINA)   

  • Online:2005-04-25 Published:2005-04-28

被引次数

29

摘要： 目的观察不同浓度毒鼠强中毒大鼠实质性器官的超微病理变化。方法采用灌胃方法使大鼠毒鼠强染毒,制作大鼠中毒模型,并以健康大鼠灌服生理盐水为对照,提取大鼠的脑皮质、脑干桥脑部、心、肝、脾、肾等实质性器官,进行超微病理观察。结果不同浓度的毒鼠强可对心肌细胞、脑神经细胞、神经纤维和肝细胞造成一定损伤,而对肾、脾组织细胞损伤不明显。脑皮质神经元细胞结构模糊,内质网轻度扩张,线粒体扩张水肿,且无明显的剂量-反应关系;脑干桥脑部可见多处弥散性软化灶,神经细胞核溶解,神经纤维细胞有坏死,血管内皮细胞肿胀,血管周围有间隙出现,脑干损伤有明显的剂量-反应关系;心肌细胞表现为不同程度的心肌细胞坏死、线粒体弥漫性崩解或肿胀、肌丝断裂溶解、肌溶灶普遍存在等变化,并呈现出明显的剂量-效应关系;肝细胞表现弥漫性肿胀,细胞模糊,肝窦狭小,内皮细胞肿胀,结构模糊不清,细胞质内线粒体明显肿胀,糖原含量减少,与中毒剂量和时间均呈明显相关性。结论脑、心、肝可能为其毒性作用的主要靶器官或靶组织。

关键词: 毒鼠强, 中毒, 毒理病理学, 超微病理变化, 透射电镜技术

Abstract: Objective To observe ultra-structural pathological changes of materiality viscera of rats poisoned by different dose of tetramine and to study the toxic mechanism. Methods Acute and subacute tetramine toxicity models were made by oral administration with different dose of tetramine. Brain, heart, liver, spleen and kidney were extracted and observed by electromicroscopic examination. Results The injuries of brain cells, cardiocytes and liver cells were induced by different dose of tetramine. These were not obviously different of the injuries of the kindy cells and spleen cells of rats poisoned by different dose of tetramine. Ultra-structural pathological changes were abserved including mitochondria slight swelling and neurolemma′s array turbulence in the brain cells, mitochondria sweeling or abolish and rupture of muscle fiber in the heart cells, mitochondria swelling and the glycogen decreased in the liver cells. Conclusion The toxic target organs of tetramine are the heart, brain and liver.

Key words: tetramine, poisoning, toxicologic pathology, ultra-structural pathological changes, tranmission electron microscope