法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (4): 280-282,285.

• 论文 • 上一篇    下一篇

PCR-SBT法检测北京地区人群HLA-DRB1遗传多态性

邓亚军,杨光,吴东颖,胡松年,李生斌,朱波峰,朱军,刘耀   

  1. 西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室,中国科学院北京基因组研究所,北京华大方瑞司法物证鉴定中心,西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室,西安交通大学卫生部法医学重点实验室 陕西西安710061;中国科学院北京基因组研究所,北京101300;北京华大方瑞司法物证鉴定中心,北京101300 ,陕西西安710061;中国科学院北京基因组研究所,北京101300 ,北京101300;北京华大方瑞司法物
  • 发布日期:2005-08-25 出版日期:2005-08-28

Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region

DENG YA-JUN1,2, YANG GUANG1,2, WU DONG-YING2, ET AL. (1.THE NATIONAL LABORATORY OF THE MINISTRY OF HEALTH FOR FORENSIC SCIENCES, XI′AN JIAOTONG UNIVERSITY, XI′AN 710016, CHINA; 2.BEIJING GENOMICS INSTITUTE, CHINESE ACADEMY OF SCIENCES, BEIJING 101300, CHI   

  • Online:2005-08-25 Published:2005-08-28

被引次数

15

摘要： 目的调查北京地区人群HLA-DRB1基因座多态性,并探讨HLA的聚合酶链反应-直接测序分型(PCR-SBT)在法医物证学中的应用价值。方法应用PCR-SBT分型方法对北京地区人群中494名健康无关个体进行HLA-DRB1基因座高分辨分型。结果检出233种HLA-DRB1基因型、102种DRB1等位基因型,基因型的分布符合Hardy-Weinberg平衡定律,该位点的观察杂合度(Ho)为0.9210,期望杂合度(He)为0.9342,多态信息量(PIC)为0.9333,匹配概率(Pm)为0.0104,个体识别率(DP)为0.9896,非父排除率(PPE)为0.7776。结论使用PCR-SBT对HLA进行精确分型在法医学领域具有重要的应用价值。

关键词: HLA-DRB1, 基因频率, PCR-SBT, 法医学

Abstract: Objective To investigate the polymorphism of HLA-DRB1 gene in a population of Beijing region and research on the application feasibility of HLA-DRB1 by PCR-SBT method. Methods Polymerase chain reaction sequence-based typing (PCR-SBT) method was used to determine HLA-DRB1 genotypes of 494 unrelated healthy people of Beijing region. Results A total of 233 different HLA-DRB1 genetypes and 102 alleles were detected. The observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), probability of matching (Pm); discrimination power (DP) and probability of paternity exclusion (PPE) of HLA-DRB1 in the samples were calculated and their values were 0.921 0,0.934 2, 0.933 3, 0.010 4, 0.989 6,and 0.777 6 respectively. Conclusion HLA-DRB1 typing by PCR-SBT is very useful in forensic science.

Key words: HLA-DRB1, PCR-SBT, DNA typing, gene frequency, forensic science