法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (3): 210-212+.

• 论文 • 上一篇    下一篇

云南汉族人群11个Y-STR基因座遗传多态性调查及法医学应用

张晓红;吴微微;唐建新;钱光立;张小明;   

  1. 广州市公安局番禺区分局,浙江省公安厅刑侦总队,广州市公安局番禺区分局,弥勒县公安局,广州市公安局番禺区分局 广东广州511400,浙江杭州310009,广东广州511400,云南弥勒652300,广东广州511400
  • 发布日期:2006-06-25 出版日期:2006-06-28

Polymorphisms of Eleven Y-chromosome STR loci and Forensic Application in Yunnan Han Population

ZHANG XIAO-HONG1,WU WEI-WEI2,TANG JIAN-XIN1,QIAN GUANG-LI3,ZHANG XIAO-MING1(1.PANYU DIVISION OF GUANGZHOU PUBLIC SECURITY BUREAU,GUANGZHOU 511400,CHINA; 2.DEPARTMENT OF CRIMINAL INVESTIGATION,ZHEJIANG PROVINCIAL PUBLIC SECURITY BUREAU,HANGZHOU 310009,CHIN   

  • Online:2006-06-25 Published:2006-06-28

被引次数

31

摘要： 目的调查11个Y-STR基因座及其单倍型在云南汉族人群中的遗传多态性分布,探讨其法医学应用价值,为法医学应用提供基础数据。方法应用Powerplex!Y系统对云南汉族201名无关男性个体进行11个Y-STR基因座的复合扩增,用ABI310型基因分析仪对扩增产物进行检测,统计其群体遗传学参数。结果Powerplex!Y系统前10个Y-STR基因座分别检出3、5、6、8、5、4、5、8、4、7个等位基因,DYS385a/b基因座检出56种单倍型;GD值最低为0.4273(DYS438),最高为0.9747(DYS385a/b);观察到11个Y-STR基因座共同构成的单倍型175种,其中有154种单倍型只出现1次,16种出现2次,5种出现3次,累计GD值为0.9984。结论11个Y-STR基因座具有较强的个体识别能力,可应用于云南地区汉族人群的个体识别与亲权鉴定。

关键词: Y染色体, 短串联重复序列(STR), 单倍型, 遗传多态性

Abstract: Objective To abtain the genetic polymorphisms data of eleven Y-chromosome STR loci in Yunnan Han population, and to evaluate its forensic application values, which will provide the base data for forensic application. Methods DYS391,DYS389Ⅰ,DYS439,DYS389Ⅱ,DYS438,DYS437,DYS19,DYS392,DYS393,DYS390 and DYS385a/b loci were amplified by using Powerplex?誖Y PCR amplification Kit and were analyzed by 310 genetic analyzer. 201 unrelated male individuals of Han population in Yunnan were investigated to determine the distributions of allele frequencies and haplotypes. Results 3,5,6,8,5,4,5,8,4,7 alleles were found across the Y-STR loci except DYS38556 haplotypes were found across DYS385a/b locus. The minimum GD value was 0.4273(DYS438),and the maximum was 0.9747(DYS385a/b). 175 haplotypes were found, of which 154 were unique, 16 were found in 2 individuals,5 were found in 3 individuals, and the observed haplotypes diversity value was 0.9984. Conclusion The 11 Y-STR loci are highly polymorphic and are suitable for forensic personal identification and paternity testing in Yunnan Han population .

Key words: Y-chromosome, short tandem repeat(STR), haplotype, genetic polymorphism