法医学杂志

法医学杂志 ›› 2009, Vol. 25 ›› Issue (4): 246-248+.

• 论文 • 上一篇    下一篇

大鼠博落回总碱中毒后心肌细胞Bcl-2、Bax蛋白表达

张德雨;孔清泉;张纯兵;朱建华;陈明娣;王澎;   

  1. 浦东新区公利医院司法鉴定所;溧水县公安局;华东政法大学刑事司法学院;皖南医学院法医系;
  • 发布日期:2009-08-25 出版日期:2009-08-28

Expression of Bcl-2 and Bax Proteins in Myocardial Cells after Macleaya Cordata Alkaloids Poisoning in Rats

ZHANG DE- YU1, KONG QING-QUAN2, ZHANG CHUN-BING3, ZHU JIAN-HUA4, CHEN MING-DI1, WANG PENG1 (1. INSTITUTE OF FORENSIC SCIENCE, GONGLI HOSPITAL OF PUDONG NEW DISTRICT, SHANGHAI 200135, CHINA; 2. LISHUI PUBLIC SECURITY BUREAU, LISHUI 211200, CHINA; 3. COLLEG   

  • Online:2009-08-25 Published:2009-08-28

被引次数

8

摘要： 目的观察大鼠博落回总碱中毒后不同时间段心肌细胞内Bcl-2和Bax蛋白表达情况,为博落回中毒鉴定提供分子生物学标志物。方法制作大鼠博落回总碱中毒实验模型,通过免疫组化染色检测技术,观测不同时间段大鼠心肌细胞内Bcl-2和Bax蛋白表达情况,并对其结果进行图像分析。结果博落回总碱中毒后不同时间段心肌细胞内Bcl-2和Bax蛋白表达不同,高于正常对照组(P<0.05)。结论博落回总碱中毒症状不明显情况下,应用免疫组织化学技术检测Bcl-2和Bax蛋白表达,可作为鉴定博落回总碱中毒的辅助手段。

关键词: 法医毒理学, 中毒, 博落回, 肌细胞, 心脏, Bcl-2蛋白, Bax蛋白, 大鼠

Abstract: Objective To observe the expression of Bcl-2 and Bax proteins in rat′s myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning. Methods Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system. Results The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05). Conclusion If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.

Key words: forensic toxicology, poisoning, Macleaya cordata, myocytes, cardiac, Bcl-2 protein, Bax protein, rats