法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (2): 157-160.DOI: 10.3969/j.issn.1004-5619.2018.02.011

• 技术与应用 • 上一篇    下一篇

联苯胺试验对血痕DNA检验的影响

金  明1，巴华杰1，朱爱华1，马  骏1，石津玮1，刘亚楠2，林子清3   

  1. 1. 常州市公安局物证鉴定所，江苏 常州 213003； 2. 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083； 3. 中国刑警学院法医学系，辽宁 沈阳 110035
  • 发布日期:2018-04-25 出版日期:2018-04-28
  • 通讯作者: 巴华杰，男，硕士，副主任法医师，主要从事法医DNA检案与研究；E-mail：bahuajie@163.com
  • 作者简介:金明（1990—），男，主要从事法医DNA检案与研究；E-mail：1017727995@qq.com

Effect of Benzidine Test on DNA Analysis of Bloodstain

JIN Ming1, BA Hua-jie1, ZHU Ai-hua1, MA Jun1, SHI Jin-wei1, LIU Ya-nan2, LIN Zi-qing3   

  1. 1. Institute of Forensic Science, Changzhou Public Security Bureau, Changzhou 213003, China; 2. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 3. Department of Forensic Medicine,　China Criminal Police College, Shenyang 110035, China
  • Online:2018-04-25 Published:2018-04-28

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摘要： 目的 探讨联苯胺试验及相关试剂对血痕DNA检验的影响。 方法 制作含1 μL静脉血的滤纸血痕970份，其中10份为对照样本，960份经联苯胺试验及相关试剂分别处理后，采用Chelex-100法和硅珠法提取DNA，AmpF?詛STRTM IdentifilerTM Plus PCR扩增试剂盒进行复合扩增，对比各组STR分型结果。　结果　联苯胺试验后立即提取DNA，硅珠法的STR基因座检出数为（3.80±1.34）个，而Chelex-100法均未获得STR分型结果；联苯胺试验后干燥处理，硅珠法有13例（21.7%）获得全部STR基因座分型结果，且STR基因座检出数［（12.90±1.49）个］远高于Chelex-100法［（4.70±1.96）个］（P<0.05）；加入冰醋酸后立即提取DNA，硅珠法的STR基因座检出数为（9.40±2.09）个，而Chelex-100法均未获得STR分型结果；只加冰醋酸后干燥处理以及只加四甲基联苯胺乙醇饱和溶液或3%过氧化氢溶液，两种方法均获得完整15个STR基因座分型结果。 结论　联苯胺试验对血痕的后续DNA检验有很大的影响，Chelex-100法不适合联苯胺试验后血痕的DNA检验，联苯胺试验后干燥处理及采用硅珠纯化的方法可有效提升联苯胺试验后血痕的STR基因座检出数。

关键词: 法医遗传学, 联苯胺试验, 血迹, DNA提取, STR分型

Abstract: Objective To explore the effect of benzidine test and related reagents on DNA analysis of bloodstain. Methods A total of 970 bloodstain filter paper samples with 1 μL venous blood were collected, and 10 of them acted as control samples. After benzidine test and related reagent processing, DNA of 960 samples was extracted by Chelex-100 and silica bead methods and then multiplex amplified by AmpF?詛STRTM IdentifilerTM Plus PCR kits. The results of STR typing were compared between different groups. Results DNA were extracted immediately after benzidine test. Totally STR loci （3.80±1.34） were detected by silica bead method, while no STR loci were obtained by Chelex-100 method. Thirteen samples （21.7%） with whole STR typing results were obtained by drying after benzidine test, and the STR locus number （12.90±1.49） which obtained by silica bead method was much higher than by Chelex-100 method （4.70±1.96） （P<0.05）. When DNA was extracted immediately after the addition of glacial acetic acid, the STR locus number was （9.40±2.09） by silica bead method, but no STR typing result was obtained by Chelex-100 method. All 15 STR loci could be obtained by only adding glacial acetic acid after drying and only adding tetramethylbenzidine alcoholization liquid or 3% hydrogen peroxide liquid. Conclusion Benzidine test has significant influence on DNA analysis of bloodstain. The Chelex-100 method is not suitable for the DNA extraction of bloodstain after benzidine test. Drying after benzidine test and silica bead methods can effectively enhance the STR locus number of bloodstain.

Key words: forensic genetics, benzidine test, blood stains, DNA extration, STR typing